氧对大鼠肝微粒体膜结构及亲脂性氮氧化物代谢的影响。

Effects of oxygen on the membrane structure and the metabolism of lipophilic nitroxide in rat liver microsomes.

作者信息

Miura Y, Utsumi H, Kashiwagi M, Hamada A

机构信息

Department of Health Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo.

出版信息

J Biochem. 1990 Oct;108(4):516-8. doi: 10.1093/oxfordjournals.jbchem.a123233.

DOI:10.1093/oxfordjournals.jbchem.a123233

PMID:1963429

Abstract

The effects of oxygen on the metabolism of lipophilic nitroxide and the membrane structure of microsomes were investigated with an ESR spectrometer equipped with a hand-made gas-permeable sampling tube. The half life of gas change in the membrane was confirmed to be 48 s by the line-width of the ESR signal. The signal intensity of lipophilic nitroxide in microsomal membranes decreased with NADPH under N2 and increased under O2. The change of signal intensity was reversible, suggesting that lipophilic nitroxide can be used as an indicator of the redox state in membranes for in vivo ESR imaging.

摘要

使用配备自制透气采样管的电子自旋共振光谱仪，研究了氧气对亲脂性氮氧化物代谢及微粒体膜结构的影响。通过电子自旋共振信号的线宽证实膜中气体变化的半衰期为48秒。在氮气条件下，微粒体膜中亲脂性氮氧化物的信号强度随烟酰胺腺嘌呤二核苷酸磷酸（NADPH）降低，在氧气条件下则升高。信号强度的变化是可逆的，这表明亲脂性氮氧化物可用作体内电子自旋共振成像中膜氧化还原状态的指示剂。