食异源鞘氨醇单胞菌的细胞及细胞提取物对醌介导的磺化偶氮染料的脱色作用
Quinone-mediated decolorization of sulfonated azo dyes by cells and cell extracts from Sphingomonas xenophaga.
作者信息
Jiao Ling, Lu Hong, Zhou Jiti, Wang Jing
机构信息
School of Environmental and Biological Science and Technology, Key Laboratory of Industrial Ecology and Environmental Engineering, Ministry of Education, Dalian University of Technology, Dalian 116024, China.
出版信息
J Environ Sci (China). 2009;21(4):503-8. doi: 10.1016/s1001-0742(08)62299-8.
The effects of various quinone compounds on the decolorization rates of sulfonated azo dyes by Sphingomonas xenophaga QYY were investigated. The results showed that anthraquinone-2-sulfonate (AQS) was the most effective redox mediator and AQS reduction was the rate-limited step of AQS-mediated decolorization of sulfonated azo dyes. Based on AQS biological toxicity tests, it was assumed that AQS might enter the cells and kill them. In the cytoplasmic extracts from strain QYY, AQS more effectively increased decolorization rates of sulfonated azo dyes than other quinone compounds. In addition, we found a NADH/FMN-dependent AQS reductase using nondenaturing polyacrylamide gel electrophoresis (Native-PAGE).
研究了各种醌类化合物对食异源鞘氨醇单胞菌QYY对偶氮染料脱色率的影响。结果表明，蒽醌-2-磺酸盐（AQS）是最有效的氧化还原介质，AQS的还原是AQS介导的磺化偶氮染料脱色的限速步骤。基于AQS生物毒性试验，推测AQS可能进入细胞并将其杀死。在菌株QYY的细胞质提取物中，AQS比其他醌类化合物更有效地提高了磺化偶氮染料的脱色率。此外，我们通过非变性聚丙烯酰胺凝胶电泳（Native-PAGE）发现了一种依赖NADH/FMN的AQS还原酶。
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