Bahri-Sahloul R, Ammar S, Fredj R B, Saguem S, Grec S, Trotin F, Skhiri F H
Faculty of Science Tunis, Campus Universitaire, 1060 Tunis El Manar, Tunisia.
Pak J Biol Sci. 2009 May 1;12(9):660-8. doi: 10.3923/pjbs.2009.660.668.
Phenolic contents of the ethyl acetate extracts prepared from floral buds and opened flowers harvested on Crataegus azarolus trees native in two localities were performed. The antioxidant activity was measured by DPPH' (2,2-diphenyl-picrylhydrazyl), ABTS+ (2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radicals scavenging using spectrophotometric method. The C. azarolus var. aronia (Willd.) Batt., producing yellow fruits, was richer in total phenols (1638.7 +/- 89.9 mg acid gallic/100 g dry weight) according to C. azarolus var. eu-azarolus Maire (1415.5 +/- 23.8 mg acid gallic/100 g dry weight), producing red ones. Ethyl acetate extract from opened flowers has less content in total phenols, proanthocyanidins and flavonoids compared to this from floral buds. Floral buds from the two C. azarolus varieties occurring in Siliana-Djebel Serdj showed the highest radical scavenging activities (2431.8 +/- 32.7 and 2267.7 +/- 22.7 micromol Trolox/100 g dry weight). Hawthorn from Tunisia contains eight antioxidants of phenolic type (chlorogenic acid, hyperoside, rutin, spiraeoside, isoquercitrin, quercetin, (-)-epicatechin and the dimer procyanidin B2). These compounds identified specially in floral bud extracts presented a strong radical-scavenging activity.
对采自两个地区的原生阿扎罗山楂树上的花芽和开放花朵所制备的乙酸乙酯提取物中的酚类成分进行了分析。采用分光光度法，通过DPPH'（2,2-二苯基-苦味酰基肼）、ABTS+（2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）自由基清除法测定抗氧化活性。与产红色果实的阿扎罗山楂变种（阿扎罗山楂，Maire）（1415.5±23.8毫克没食子酸/100克干重）相比，产黄色果实的阿扎罗山楂变种（aronia（Willd.）Batt.）的总酚含量更高（1638.7±89.9毫克没食子酸/100克干重）。与花芽中的乙酸乙酯提取物相比，开放花朵中的乙酸乙酯提取物的总酚、原花青素和黄酮类化合物含量较低。来自锡利亚纳-杰贝勒塞尔德的两个阿扎罗山楂变种的花芽表现出最高的自由基清除活性（2431.8±32.7和2267.7±22.7微摩尔 Trolox/100克干重）。突尼斯山楂含有八种酚类抗氧化剂（绿原酸、金丝桃苷、芦丁、绣线菊苷、异槲皮苷、槲皮素、（-）-表儿茶素和二聚体原花青素B2）。这些在花芽提取物中特别鉴定出的化合物具有很强的自由基清除活性。
