Bernatoniene Jurga, Masteikova Rūta, Majiene Daiva, Savickas Arūnas, Kevelaitis Egidijus, Bernatoniene Rūta, Dvorácková Katerina, Civinskiene Genuvaite, Lekas Raimundas, Vitkevicius Konradas, Peciūra Rimantas
Department of Drug Technology and Social Pharmacy, Kaunas University of Medicine, Kaunas, Lithuania.
Medicina (Kaunas). 2008;44(9):706-12.
The aim of this study was to investigate antiradical activity of aqueous and ethanolic hawthorn fruit extracts, their flavonoids, and flavonoid combinations.
Total amount of phenolic compounds and the constituents of flavonoids were determined using a high-performance liquid chromatography. The antioxidant activity of Crataegus monogyna extracts and flavonoids (chlorogenic acid, hyperoside, rutin, quercetin, vitexin-2O-rhamnoside, epicatechin, catechin, and procyanidin B(2)) quantitatively was determined using the method of spectrophotometry (diphenyl-1-picrylhydrazyl (DPPH.) radical scavenging assay and 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid)(ABTS.+) radical cation decolorization assay). The level of tyrosine nitration inhibition was determined using a high-performance liquid chromatography.
Ethanolic hawthorn fruit extract contained 182+/-4 mg/100 mL phenolic compounds, i.e. threefold more, as compared to aqueous extract. The antioxidant activity according to DPPH. reduction in the ethanolic extracts was higher 2.3 times (P<0.05). The ABTS.+ technique showed that the effect of ethanolic extracts was by 2.5 times stronger than that of aqueous extracts. Tyrosine nitration inhibition test showed that the effect of ethanolic extracts was by 1.4 times stronger than that of aqueous extracts. The investigation of the antiradical activity of the active constituents in aqueous and ethanolic extracts revealed that epicatechin and catechin contribute to radical-scavenging properties more than other components. Procyanidin B(2) only insignificantly influenced the antiradical activity of the extracts.
Both aqueous and ethanolic hawthorn extracts had antiradical activity, but ethanolic extract had stronger free radical-scavenging properties, compared to the aqueous extract. The antioxidant activity of the studied preparations was mostly conditioned by epicatechin and catechin. The individual constituents of both extracts had weaker free radical-scavenging properties than the combination of these substances did.
本研究的目的是研究山楂果实水提取物和乙醇提取物、其黄酮类化合物以及黄酮类化合物组合的抗自由基活性。
使用高效液相色谱法测定酚类化合物的总量和黄酮类化合物的成分。采用分光光度法（二苯基-1-苦基肼（DPPH.）自由基清除试验和2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS. +）自由基阳离子脱色试验）定量测定山楂提取物和黄酮类化合物（绿原酸、金丝桃苷、芦丁、槲皮素、牡荆素-2-O-鼠李糖苷、表儿茶素、儿茶素和原花青素B(2)）的抗氧化活性。使用高效液相色谱法测定酪氨酸硝化抑制水平。
乙醇山楂果实提取物含有182±4mg/100mL酚类化合物，即比水提取物多两倍。根据DPPH.还原法，乙醇提取物的抗氧化活性高2.3倍（P<0.05）。ABTS. +技术表明，乙醇提取物的效果比水提取物强2.5倍。酪氨酸硝化抑制试验表明，乙醇提取物的效果比水提取物强1.4倍。对水提取物和乙醇提取物中活性成分的抗自由基活性研究表明，表儿茶素和儿茶素比其他成分对自由基清除特性的贡献更大。原花青素B(2)对提取物的抗自由基活性影响不大。
山楂水提取物和乙醇提取物均具有抗自由基活性，但与水提取物相比，乙醇提取物具有更强的自由基清除特性。所研究制剂的抗氧化活性主要由表儿茶素和儿茶素决定。两种提取物的单个成分的自由基清除特性比这些物质的组合弱。
