Rosenblatt Robert, Valdman Alexander, Cheng Liang, Lopez-Beltran Antonio, Montironi Rodolfo, Ekman Peter, Egevad Lars
Department of Oncology-Pathology, Karolinska Institute, Stockholm, Sweden.
Anal Quant Cytol Histol. 2009 Jun;31(3):137-42.
To investigate the prognostic value of endothelin-1 (ET-1), a vasoconstrictor involved in differentiation and growth of cancer, in prostate cancer.
A tissue microarray was constructed of 287 prostate cancers from radical prostatectomy (RP) specimens with median follow-up of 48.9 months. Slides were immunostained for ET-1. Intensity and extent of immunoreactivity and their product (IRp) were evaluated. We separately arrayed benign prostatic tissue, atrophy, high grade prostatic intraepithelial neoplasia (HGPIN) and prostate cancer from 40 men.
ET-1 expression was stronger in both HGPIN and cancer than in benign tissue (p < 0.001). The intensity and the IRp of ET-1 predicted biochemical recurrence (p < 0.001 and p = 0.044, respectively), while the staining extent showed no significant correlation with outcome (p = 0.68). Recurrence-free survival in patients with strong ET-1 staining was shorter than in those with weaker expression (hazard ratio [CI 95%] 2.44 [1.55-3.84], p < 0.001). In a multivariate analysis including ET-1 expression, preoperative serum prostate-specific antigen (PSA), extraprostatic extension, margin status, seminal vesicle invasion and Gleason score, only PSA, margins and Gleason score remained significant.
ET-1 is overexpressed in localized prostate cancer and predicts outcome after RP in univariate analysis.
研究内皮素-1（ET-1），一种参与癌症分化和生长的血管收缩因子，在前列腺癌中的预后价值。
构建组织芯片，包含287例来自根治性前列腺切除术（RP）标本的前列腺癌，中位随访时间为48.9个月。载玻片进行ET-1免疫染色。评估免疫反应强度、范围及其乘积（IRp）。我们分别对40名男性的良性前列腺组织、萎缩、高级别前列腺上皮内瘤变（HGPIN）和前列腺癌进行了阵列分析。
ET-1在HGPIN和癌症中的表达均强于良性组织（p < 0.001）。ET-1的强度和IRp可预测生化复发（分别为p < 0.001和p = 0.044），而染色范围与预后无显著相关性（p = 0.68）。ET-1染色强的患者无复发生存期短于表达较弱的患者（风险比[95%CI] 2.44 [1.55 - 3.84]，p < 0.001）。在包括ET-1表达、术前血清前列腺特异性抗原（PSA）、前列腺外侵犯、切缘状态、精囊侵犯和Gleason评分的多因素分析中，只有PSA、切缘和Gleason评分仍然具有显著性。
ET-1在局限性前列腺癌中过度表达，在单因素分析中可预测RP后的预后。
