Pinard R, Richards J G, Lanoir J
C.N.R.S., Laboratoire de Neurobiologie, Département Voies et Neurotransmission Centrales, Marseilles, France.
Neurosci Lett. 1990 Dec 11;120(2):212-6. doi: 10.1016/0304-3940(90)90041-7.
The monoclonal antibody bd-17, which recognizes the beta 2 and beta 3-subunits of GABAA/benzodiazepine receptors, was used to determine the cellular and subcellular localization of receptor-like immunoreactivity in the superficial gray layer of the rat superior colliculus. In numerous dendrites, very strong immunostaining was present in the cytoplasm and on the postsynaptic dendritic membrane of synaptic junctions. The extrasynaptic portion of the dendritic membrane also very often showed [beta 2 + beta 3]-like immunoreactivity. However, due to methodological limitations, it could not be stated with certainty whether presynaptic beta 2- and beta 3-subunits of GABAA/benzodiazepine receptors actually occur in this mesencephalic visual structure. In conclusion, these results strongly suggest that synaptic and non-synaptic GABAA/benzodiazepine receptors are present in the superficial gray layer of the rat superior colliculus. These receptors may modulate neuronal cell activity in different ways, depending on their location.
单克隆抗体bd - 17可识别GABAA/苯二氮䓬受体的β2和β3亚基，用其来确定大鼠上丘浅层灰质中受体样免疫反应性的细胞和亚细胞定位。在众多树突中，突触连接处的细胞质和突触后树突膜上存在非常强的免疫染色。树突膜的突触外部分也经常显示出[β2 + β3]样免疫反应性。然而，由于方法学上的限制，无法确定GABAA/苯二氮䓬受体的突触前β2和β3亚基是否真的存在于这个中脑视觉结构中。总之，这些结果有力地表明，大鼠上丘浅层灰质中存在突触和非突触GABAA/苯二氮䓬受体。这些受体可能根据其位置以不同方式调节神经元细胞活性。
