Zhao Yi, Deng Xin, Wang Qiang
Department of Surgery, Sheng Jing Hospital, China Medical University, Shenyang, Liaoning, 110004, P.R. China.
Ai Zheng. 2009 Jun;28(6):593-601.
The proapoptotic gene Smac/DIABLO interacts with inhibitor of apoptosis protein Livin, which may contribute to regulating the impetus and progression of canceration. This study was to investigate the expression of Livin and Smac/DIABLO, and their correlations to the clinicopathological features in gastric adenocarcinomas.
The expression levels of Livin and Smac/DIABLO mRNA in 75 gastric carcinoma specimens, 20 normal gastric tissues and 20 para-cancerous tissues were detected using the SYBR green real-time fluorescent quantitative reverse transcriptase PCR. The protein expression and location of Livin and Smac/DIABLO were measured by western blot and immunohistochemistry (SP method).
The expression of Livin mRNA was significantly up-regulated in gastric carcinomas (6.374+/-4.759) compared with the control. The expression of Livin was higher in poorly differentiated gastric carcinomas and carcinomas with lymph node metastases than in highly differentiated gastric carcinomas and carcinomas without lymph node metastases. (Chi2=9.60, P<0.01; Chi2=5.51, P<0.05). The expression of Livin mRNA had no correlation to tumor site, and the invasion depth and TNM stage (P >0.05). In contrast, no expression of Livin was detected in normal and para-cancerous gastric tissues. The protein expression of Livin was consistent with that of its mRNA expression. The expression level of Smac/DIABLO mRNA was lower in gastric carcinomas (0.731+/-0.420) than in normal gastric tissues (1.104+/-0.276) and para-cancerous tissues (1.061+/-0.737), but there was no significant difference (P>0.05). The expression of Smac/DIABLO mRNA was not correlated to clinicopathological characters of gastric adenocarcinoma. The protein expression of Smac/DIABLO was not completely consistent with that of its mRNA expression. The expression of Smac/DIABLO was different between intestinal type gastric carcinoma (84.38%) and diffuse type gastric carcinoma (60.65%) (P<0.05).
The expression and correlation of Livin and Smac/DIABLO vary in different stages and pathological types of gastric carcinoma. Overexpression of Livin is closely related to differentiation and metastases of gastric carcinoma.
促凋亡基因Smac/DIABLO与凋亡抑制蛋白Livin相互作用，这可能有助于调控癌变的动力和进程。本研究旨在探讨Livin和Smac/DIABLO在胃腺癌中的表达及其与临床病理特征的相关性。
采用SYBR green实时荧光定量逆转录聚合酶链反应检测75例胃癌标本、20例正常胃组织和20例癌旁组织中Livin和Smac/DIABLO mRNA的表达水平。通过蛋白质印迹法和免疫组织化学（SP法）检测Livin和Smac/DIABLO的蛋白表达及定位。
与对照组相比，胃癌组织中Livin mRNA的表达显著上调（6.374±4.759）。低分化胃癌和有淋巴结转移的胃癌中Livin的表达高于高分化胃癌和无淋巴结转移的胃癌（χ2=9.60，P<0.01；χ2=5.51，P<0.05）。Livin mRNA的表达与肿瘤部位、浸润深度和TNM分期无关（P>0.05）。相反，在正常胃组织和癌旁胃组织中未检测到Livin的表达。Livin的蛋白表达与其mRNA表达一致。胃癌组织中Smac/DIABLO mRNA的表达水平（0.731±0.420）低于正常胃组织（1.104±0.276）和癌旁组织（1.061±0.737），但差异无统计学意义（P>0.05）。Smac/DIABLO mRNA的表达与胃腺癌的临床病理特征无关。Smac/DIABLO的蛋白表达与其mRNA表达不完全一致。肠型胃癌（84.38%）和弥漫型胃癌（60.65%）中Smac/DIABLO的表达不同（P<0.05）。
Livin和Smac/DIABLO在胃癌的不同阶段和病理类型中的表达及相关性存在差异。Livin的过表达与胃癌的分化和转移密切相关。
