用于汞形态分析的冷蒸气发生接口——毛细管电泳与电热石英管炉原子吸收光谱联用：汞和甲基汞的测定

Cold vapor generation interface for mercury speciation coupling capillary electrophoresis with electrothermal quartz tube furnace atomic absorption spectrometry: determination of mercury and methylmercury.

作者信息

Deng Biyang, Xiao Yan, Xu Xiangshu, Zhu Pingchuan, Liang Shaojun, Mo Weiming

机构信息

Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, China.

出版信息

Talanta. 2009 Oct 15;79(5):1265-9. doi: 10.1016/j.talanta.2009.05.037. Epub 2009 May 28.

DOI:10.1016/j.talanta.2009.05.037

PMID:19635357

Abstract

A novel on-line coupled capillary electrophoresis (CE) cold vapor generation (CVG) with electrothermal quartz tube furnace atomic absorption spectrometry (EQTF-AAS) system for mercury speciation has been developed. The mercury species (inorganic mercury and methylmercury) were completely separated by CE in a 80 cm length x 100 microm i.d. fused-silica capillary at 20 kV and using a buffer of 100 mM boric acid and 10% (v/v) methanol (pH 8.30). The effects of the inner diameter of quartz tube, the acidity of HCl, the NaBH(4) concentration and N(2) flow rate on Hg signal intensity were investigated. Speciation of mercury was highlighted using CE-CVG-EQTF-AAS. The detection limits of methylmercury and mercury were 0.035 and 0.027 microg mL(-1), respectively. The precisions (RSDs) of peak height for six replicate injections of a mixture of 10 microg mL(-1) (as Hg) were better than 4%. The interface was used for speciation analysis of mercury in dry goldfish muscle.

摘要

已开发出一种用于汞形态分析的新型在线耦合毛细管电泳（CE）冷蒸气发生（CVG）与电热石英管炉原子吸收光谱法（EQTF-AAS）系统。汞形态（无机汞和甲基汞）在80 cm长×100μm内径的熔融石英毛细管中，于20 kV电压下，使用100 mM硼酸和10%（v/v）甲醇（pH 8.30）的缓冲液，通过CE实现了完全分离。研究了石英管内径、HCl酸度、NaBH₄浓度和N₂流速对Hg信号强度的影响。使用CE-CVG-EQTF-AAS突出了汞的形态分析。甲基汞和汞的检测限分别为0.035和0.027μg mL⁻¹。对10μg mL⁻¹（以Hg计）混合物进行六次重复进样的峰高精密度（相对标准偏差，RSDs）优于4%。该接口用于干金鱼肌肉中汞的形态分析。