Department of Cardiovascular Surgery, Xijing Hospital, Fourth Military Medical University, 17 Changle West Road, Xi'an 710032, China.
J Biotechnol. 2009 Sep 25;143(4):268-73. doi: 10.1016/j.jbiotec.2009.07.015. Epub 2009 Jul 25.
In recent years, utilizing nanoparticles as delivery system for drug targeting delivery has raised increasing interest. In this study, glycidyl methacrylate derivatized dextran (Dex-GMA) nanoparticles encapsulating basic fibroblast growth factor (bFGF) have been prepared inside the aqueous cores of sodium bis(2-ethylhexyl) sulfosuccinate (AOT)/n-hexane reverse micelles. These nanoparticles were identified to be uniformly spherical in shape with an average size of 109.57+/-2.09 nm. And 90.2% of the nanoparticles were in a narrow size range of 80-110 nm. The release of bFGF from the nanoparticles is completely and sustained as long as 35 days. The impact of the nanoparticles on mouse bone marrow mesenchymal stem cells (BMSCs) was assessed with cell cytotoxicity/viability and adhesion assay. Those studies show that the Dex-GMA nanoparticles prepared by water-in-oil micro-emulsion systems with aprotic solvent adding are novel, effective and biocompatible delivery system for bioactive protein.
近年来，利用纳米粒子作为药物靶向传递的递送系统引起了越来越多的关注。在这项研究中，利用丙烯酰氧基甲基葡萄糖苷（Dex-GMA）修饰的葡聚糖（Dex-GMA）纳米粒子在琥珀酸双（2-乙基己基）酯磺酸钠（AOT）/正己烷反胶束的水核内包封碱性成纤维细胞生长因子（bFGF）。这些纳米粒子被鉴定为形状均匀的球形，平均粒径为 109.57+/-2.09nm。并且 90.2%的纳米粒子处于 80-110nm 的窄粒径范围内。bFGF 从纳米粒子中的释放是完全和持续的，长达 35 天。通过细胞毒性/活力和黏附试验评估了纳米粒子对小鼠骨髓间充质干细胞（BMSCs）的影响。这些研究表明，在添加非质子溶剂的油包水微乳液体系中制备的 Dex-GMA 纳米粒子是一种新型、有效和生物相容的生物活性蛋白传递系统。
