Department of Cardiovascular Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
Drug Dev Ind Pharm. 2009 Dec;35(12):1419-29. doi: 10.3109/03639040902988558.
To develop a novel efficient nanoparticulate carrier loaded with basic fibroblast growth factor (bFGF).
Gelatin and glycidyl methacrylate-derivatized dextran (dex-GMA) were cross-linked and polymerized to form interpenetrating polymeric networks. The properties of the nanoparticles (NPs) were investigated as a function of the degree of dex-GMA substitution and the concentration of gelatin used in the preparation of the hydrogels. The morphology was observed with scanning eletromicroscopy and transmission eletromicroscopy. The swelling, degradation, and entrapment efficiency were also determined by dynamic evaluation methods in vitro. The protein release ratio and in vitro release kinetics were evaluated by routine procedure, and the biological activity of bFGF-loaded NPs was studied by cell proliferation assay, cell attachment, and cell function.
The NPs have a particle size of 320 +/- 20 nm. bFGF was entrapped in the nanoparticles quantitatively (the encapsulation efficiency, 89.6 +/- 0.9%). The bFGF in vitro release kinetics fitted to zero-order and Higuchi equations. Proliferation assay, attachment assay, and western blot showed that bFGF NPs had good biological effects on cultured bone marrow mesenchymal stem cells and could achieve a much longer action time than bFGF solution.
These results suggested that a novel biodegradable dex-GMA/gelatin hydrogel NPs loaded with bFGF could be successfully developed from both dextran- and gelatin-based biomaterials.
研制一种新型高效载碱性成纤维细胞生长因子（bFGF）的纳米颗粒载体。
将明胶与甲基丙烯酰化葡聚糖（dex-GMA）交联聚合，形成互穿聚合物网络。研究了纳米颗粒（NPs）的性能，考察了 dex-GMA 取代度和水凝胶制备中明胶浓度的变化。采用扫描电镜和透射电镜观察形貌。采用动态评价方法体外测定了膨胀度、降解度和包封效率。采用常规方法评价蛋白释放率和体外释放动力学，通过细胞增殖试验、细胞黏附和细胞功能研究负载 bFGF 的 NPs 的生物学活性。
NP 的粒径为 320±20nm。bFGF 定量包封于纳米颗粒中（包封效率 89.6±0.9%）。bFGF 的体外释放动力学符合零级和 Higuchi 方程。增殖试验、黏附试验和 Western blot 表明，bFGF NPs 对培养的骨髓间充质干细胞具有良好的生物学效应，作用时间明显长于 bFGF 溶液。
这些结果表明，新型可生物降解的基于葡聚糖和明胶的生物材料载 bFGF 的 dex-GMA/明胶水凝胶 NPs 可成功研制。
