Liew F M, Yamanishi K, Kishimoto S, Yasuno H
Department of Dermatology, Kyoto Prefectural University of Medicine, Japan.
J Dermatol Sci. 1990 Sep;1(5):369-72. doi: 10.1016/0923-1811(90)90594-4.
We have developed a method for differential detection of human papillomavirus type 6 and 11 with selective amplification of a segment of the viral DNAs by the polymerase chain reaction. The target sequence for amplification was a 134-bp (bp 206-339) in the E6 open reading frame of HPV 6 and 11 DNAs. DNA extracted from a paraffin-section or a minute fresh biopsy specimen of condyloma acuminatum was amplified and hybridized with an oligonucleotide probe specific for HPV 6 or 11 DNA. The method was applied to analyse 5 cases of genital condyloma acuminatum, and as a result, HPV 6 and 11 DNAs were detected in two and one case, respectively. This method is useful for differential diagnosis of HPV 6 and 11 infection.
我们开发了一种通过聚合酶链反应选择性扩增病毒DNA片段来鉴别检测人乳头瘤病毒6型和11型的方法。扩增的靶序列是HPV 6和11型DNA的E6开放阅读框中的一段134bp（碱基对206 - 339）。从尖锐湿疣的石蜡切片或微小新鲜活检标本中提取的DNA进行扩增，并与针对HPV 6或11型DNA的寡核苷酸探针杂交。该方法应用于分析5例生殖器尖锐湿疣，结果分别在2例和1例中检测到HPV 6和11型DNA。该方法对HPV 6和11型感染的鉴别诊断有用。
