Suo Zhanwei, Liu Ye, Ferreri Miro, Zhang Tao, Liu Zhongjie, Mu Xiang, Han Bo
College of Veterinary Medicine, China Agricultural University, Beijing 100193, PR China.
J Ethnopharmacol. 2009 Sep 25;125(3):404-9. doi: 10.1016/j.jep.2009.07.023. Epub 2009 Jul 25.
Matrine (MT) is a main active ingredient of Sophora flavescens roots, which is used in Traditional Chinese Medicine (TCM) for the treatment of inflammations like enteritis, hepatitis and atopic dermatitis.
Aim of the study is to gain insight into the effects of MT on nitric oxide (NO) release, intracellular NO production, and endothelial nitric oxide synthase (eNOS) level in second generation rat intestinal microvascular endothelial cells (RIMECs). Moreover, the effects of MT on soluble intercellular adhesion molecule-1 (sICAM-1), interleukin-6 (IL-6) and interleukin-8 (IL-8) production induced by lipopolysaccharide (LPS) in these cells were evaluated.
Isolated and identified RIMECs cultures were exposed to different concentrations of matrine, and changes in extra- and intracellular NO concentrations were measured in dependance of time by Griess reaction or DAF-FM diacetate. Obtained cell cultures were solitude treated with lypopolysaccharide (LPS) or combined with MT to observe impacts on sICAM-1, IL-6 and IL-8 concentration in culture supernatants by ELISA.
Matrine dose-dependently increased the concentration of NO in culture supernatant of RIMECs. Exposure of MT resulted in a steady intracellular NO increase pattern under different concentrations with different values and has an increasing effect on eNOS concentration at a long time exposure. Additionally, matrine reduced the increasing effect of LPS on the production of IL-6, IL-8, and sICAM-1 in RIMECs.
These results show that matrine may serve as a protective agent against tissue damage in inflammation by improving NO-dependent vasomotion and inhibiting inflammatory cytokines induced by LPS.
苦参碱（MT）是苦参根的主要活性成分，在传统中药中用于治疗肠炎、肝炎和特应性皮炎等炎症。
本研究旨在深入了解苦参碱对第二代大鼠肠道微血管内皮细胞（RIMECs）中一氧化氮（NO）释放、细胞内NO生成以及内皮型一氧化氮合酶（eNOS）水平的影响。此外，还评估了苦参碱对这些细胞中脂多糖（LPS）诱导的可溶性细胞间黏附分子-1（sICAM-1）、白细胞介素-6（IL-6）和白细胞介素-8（IL-8）生成的影响。
将分离并鉴定的RIMECs培养物暴露于不同浓度的苦参碱中，通过格里斯反应或二乙酸荧光素（DAF-FM diacetate）根据时间测量细胞外和细胞内NO浓度的变化。将获得的细胞培养物单独用脂多糖（LPS）处理或与苦参碱联合处理，通过酶联免疫吸附测定（ELISA）观察对培养上清液中sICAM-1、IL-6和IL-8浓度的影响。
苦参碱剂量依赖性地增加了RIMECs培养上清液中NO的浓度。在不同浓度下，苦参碱暴露导致细胞内NO呈稳定增加模式，且长时间暴露时对eNOS浓度有增加作用。此外，苦参碱降低了LPS对RIMECs中IL-6、IL-8和sICAM-1生成的增加作用。
这些结果表明，苦参碱可能通过改善NO依赖性血管运动和抑制LPS诱导的炎性细胞因子，作为炎症中组织损伤的保护剂。
