Castorena-Cortés G, Roldán-Carrillo T, Zapata-Peñasco I, Reyes-Avila J, Quej-Aké L, Marín-Cruz J, Olguín-Lora P
Instituto Mexicano del Petróleo, Eje Central Lázaro Cárdenas 152, 07730 México, DF, Mexico.
Bioresour Technol. 2009 Dec;100(23):5671-7. doi: 10.1016/j.biortech.2009.06.050. Epub 2009 Jul 26.
Microcosm assays and Taguchi experimental design was used to assess the biodegradation of an oil sludge produced by a gas processing unit. The study showed that the biodegradation of the sludge sample is feasible despite the high level of pollutants and complexity involved in the sludge. The physicochemical and microbiological characterization of the sludge revealed a high concentration of hydrocarbons (334,766+/-7001 mg kg(-1) dry matter, d.m.) containing a variety of compounds between 6 and 73 carbon atoms in their structure, whereas the concentration of Fe was 60,000 mg kg(-1) d.m. and 26,800 mg kg(-1) d.m. of sulfide. A Taguchi L(9) experimental design comprising 4 variables and 3 levels moisture, nitrogen source, surfactant concentration and oxidant agent was performed, proving that moisture and nitrogen source are the major variables that affect CO(2) production and total petroleum hydrocarbons (TPH) degradation. The best experimental treatment yielded a TPH removal of 56,092 mg kg(-1) d.m. The treatment was carried out under the following conditions: 70% moisture, no oxidant agent, 0.5% of surfactant and NH(4)Cl as nitrogen source.
采用微观世界试验和田口实验设计来评估天然气处理装置产生的油泥的生物降解情况。研究表明，尽管该油泥污染物含量高且成分复杂，但对其进行生物降解是可行的。油泥的物理化学和微生物特性表明，其碳氢化合物浓度很高（334,766±7001毫克/千克干物质），结构中含有6至73个碳原子的各种化合物，而铁的浓度为60,000毫克/千克干物质，硫化物浓度为26,800毫克/千克干物质。进行了包含4个变量（水分、氮源、表面活性剂浓度和氧化剂）和3个水平的田口L(9)实验设计，结果证明水分和氮源是影响二氧化碳产生和总石油烃（TPH）降解的主要变量。最佳实验处理使TPH去除量达到56,092毫克/千克干物质。该处理是在以下条件下进行的：70%的水分、无氧化剂、0.5%的表面活性剂以及以氯化铵作为氮源。
