Raza Zulfiqar Ali, Ahmad Naseer, Kamal Shahid
Chemistry Research Laboratory, National Textile University, Faisalabad-37610, Pakistan.
College of Statistical and Actuarial Sciences, University of the Punjab, Lahore, Pakistan.
Biotechnol Rep (Amst). 2014 Jun 25;3:86-94. doi: 10.1016/j.btre.2014.06.007. eCollection 2014 Sep.
The present paper envisages the multi-response optimization of certain process parameters like total sugars concentration, C/N ratio and incubation time on rhamnolipid yield, surface tension reduction, biomass formation and substrate utilization, in rhamnolipid production by a mutant strain grown on clarified blackstrap molasses, under L orthogonal array in Taguchi design. The results have been analyzed by using grey relational analysis for the identification of an optimum level of process parameters. The validity of the results was checked though confirmation experiment, under grey relational analysis. Subsequently, the rhamnolipid yield improved from 1.45 to 1.50 g/L, substrate utilization reduced from 26 to 14% (w/v) and lesser biomass was formed. Moreover, the volumetric productivity of the process improved from 0.0086 to 0.0208 g/L/h by 142%. Furthermore, using analysis of variance method, significant contributions of process parameters were determined.
本文设想在田口设计的L正交阵列下，对以澄清的黑strap糖蜜为培养基生长的突变菌株生产鼠李糖脂过程中的某些工艺参数（如总糖浓度、碳氮比和培养时间）进行多响应优化，这些参数对鼠李糖脂产量、表面张力降低、生物量形成和底物利用有影响。通过灰色关联分析对结果进行分析，以确定工艺参数的最佳水平。通过在灰色关联分析下进行的验证实验检查了结果的有效性。随后，鼠李糖脂产量从1.45克/升提高到1.50克/升，底物利用率从26%（w/v）降至14%，生物量形成减少。此外，该过程的体积生产率从0.0086克/升/小时提高到0.0208克/升/小时，提高了142%。此外，使用方差分析法确定了工艺参数的显著贡献。
