Zhang G Q, Sun J, Wang H X, Ng T B
State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing, China.
Acta Biochim Pol. 2009;56(3):415-21. Epub 2009 Jul 27.
Little was known about biological activities of compounds from the medicinal mushroom of the genus Pholiota. A lectin from the Pholiota adiposa has now been isolated and its properties tested. The isolation procedure included ion exchange chromatography on DEAE-cellulose and CM-cellulose, and fast protein liquid chromatography-gel filtration (FPLC) on Superdex 75. The lectin was composed of two identical subunits, each with a molecular mass of 16 kDa. Its N-terminal amino-acid sequence showed little similarity to sequences of other Agaricales lectins. The hemagglutinating activity of the lectin was stable at temperatures up to 50 degrees C, and in NaOH and HCl solutions with concentrations less than 25 mM. It was inhibited by inulin (12.5-200 mM), but enhanced by Cu(2+) (6.25-25 mM), Fe(2+) (12.5-25 mM), and Al(3+) (6.25-25 mM) ions. The lectin showed antiproliferative activity toward hepatoma Hep G2 cells and breast cancer MCF7 cells with an IC(50) of 2.1 microM and approximately 3.2 microM, respectively. It exhibited HIV-1 reverse transcriptase inhibitory activity with an IC(50) of 1.9 microM. When compared with P. aurivella lectin, the only Pholiota lectin published to date, P. adiposa lectin differs in chromatographic behavior, molecular mass, N-terminal sequence, and effect of cations on hemagglutinating activity. In the case of the lectin from P. aurivella, its antifungal, antiproliferative, and HIV-1 reverse transcriptase inhibitory activities have not been determined.
人们对鳞伞属药用蘑菇中化合物的生物活性了解甚少。目前已从黄伞中分离出一种凝集素并对其性质进行了测试。分离过程包括在DEAE - 纤维素和CM - 纤维素上进行离子交换色谱，以及在Superdex 75上进行快速蛋白质液相色谱 - 凝胶过滤（FPLC）。该凝集素由两个相同的亚基组成，每个亚基的分子量为16 kDa。其N端氨基酸序列与其他伞菌目凝集素的序列几乎没有相似性。该凝集素的血凝活性在高达50摄氏度的温度下以及在浓度低于25 mM的NaOH和HCl溶液中稳定。它被菊粉（12.5 - 200 mM）抑制，但被Cu(2+)（6.25 - 25 mM）、Fe(2+)（12.5 - 25 mM）和Al(3+)（6.25 - 25 mM）离子增强。该凝集素对肝癌Hep G2细胞和乳腺癌MCF7细胞具有抗增殖活性，IC(50)分别为2.1 microM和约3.2 microM。它表现出HIV - 1逆转录酶抑制活性，IC(50)为1.9 microM。与迄今为止唯一已发表的鳞伞凝集素——金黄鳞伞凝集素相比，黄伞凝集素在色谱行为、分子量、N端序列以及阳离子对血凝活性的影响方面存在差异。就金黄鳞伞凝集素而言，其抗真菌、抗增殖和HIV - 1逆转录酶抑制活性尚未确定。
