State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100193, China.
Carbohydr Res. 2011 Nov 29;346(16):2576-81. doi: 10.1016/j.carres.2011.09.005. Epub 2011 Sep 16.
The isolation of a dimeric 29.8-kDa lectin (LFL) from dried Lactarius flavidulus fruit bodies is reported herein. The chromatographic procedure utilized comprised anion-exchange chromatography on DEAE-cellulose, cation-exchange chromatography on CM-cellulose, anion-exchange chromatography on SP-Sepharose, and gel filtration by fast protein liquid chromatography on Superdex 75. The hemagglutinating activity of LFL was inhibited by a variety of simple sugars, such as lactose, p-nitrophenyl α-D-glucopyranoside, p-nitrophenyl β-D-glucopyranoside and inositol, and by the polysaccharide inulin. The activity of LFL was stable up to 40°C. There was a precipitous drop in activity when the temperature was elevated to 50°C. Hemagglutinating activity was retained when LFL was exposed to 6.25-12.5 mM HCl and NaOH. The activity was potently inhibited by Fe(2+) and Fe(3+) ions, and slightly inhibited by Al(3+) and Mn(2+) ions. LFL suppressed the proliferation of hepatoma (HepG2) and leukemic (L1210) cells with an IC(50) of 8.90 and 6.81 μM, respectively. It inhibited the activity of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) with an IC(50) of 5.68 μM. However, LFL did not exhibit antifungal activity.
本文报道了从干燥的乳菇属真菌子实体中分离出一种二聚体 29.8kDa 凝集素（LFL）。所采用的色谱程序包括 DEAE-纤维素阴离子交换色谱、CM-纤维素阳离子交换色谱、SP-Sepharose 阴离子交换色谱和 Superdex 75 快速蛋白液相色谱凝胶过滤。LFL 的血凝活性被各种简单糖如乳糖、对硝基苯-α-D-吡喃葡萄糖苷、对硝基苯-β-D-吡喃葡萄糖苷和肌醇以及多糖菊粉所抑制。LFL 的活性在 40°C 时稳定。当温度升高到 50°C 时，活性急剧下降。当 LFL 暴露于 6.25-12.5mM HCl 和 NaOH 时，其血凝活性得以保留。Fe(2+)和 Fe(3+)离子强烈抑制其活性，而 Al(3+)和 Mn(2+)离子则轻微抑制其活性。LFL 对肝癌（HepG2）和白血病（L1210）细胞的增殖具有抑制作用，IC50 分别为 8.90 和 6.81μM。它抑制人类免疫缺陷病毒 1 型（HIV-1）逆转录酶（RT）的活性，IC50 为 5.68μM。然而，LFL 没有表现出抗真菌活性。
