Doran Michael Robert, Mills Richard James, Parker Anthony James, Landman Kerry Anne, Cooper-White Justin John
Australian Institute for Bioengineering and Nanotechnology, University of Queensland, Queensland 4072, Australia.
Lab Chip. 2009 Aug 21;9(16):2364-9. doi: 10.1039/b900791a. Epub 2009 May 14.
Studying the rate of cell migration provides insight into fundamental cell biology as well as a tool to assess the functionality of synthetic surfaces and soluble environments used in tissue engineering. The traditional tools used to study cell migration include the fence and wound healing assays. In this paper we describe the development of a microchannel based device for the study of cell migration on defined surfaces. We demonstrate that this device provides a superior tool, relative to the previously mentioned assays, for assessing the propagation rate of cell wave fronts. The significant advantage provided by this technology is the ability to maintain a virgin surface prior to the commencement of the cell migration assay. Here, the device is used to assess rates of mouse fibroblasts (NIH 3T3) and human osteosarcoma (SaOS2) cell migration on surfaces functionalized with various extracellular matrix proteins as a demonstration that confining cell migration within a microchannel produces consistent and robust data. The device design enables rapid and simplistic assessment of multiple repeats on a single chip, where surfaces have not been previously exposed to cells or cellular secretions.
研究细胞迁移速率不仅有助于深入了解基础细胞生物学，还能为评估组织工程中使用的合成表面和可溶性环境的功能提供一种工具。用于研究细胞迁移的传统工具包括栅栏实验和伤口愈合实验。在本文中，我们描述了一种基于微通道的装置的开发，用于研究细胞在特定表面上的迁移。我们证明，相对于上述实验，该装置为评估细胞波前的传播速率提供了一种更优越的工具。该技术的显著优势在于能够在细胞迁移实验开始前保持表面未受污染。在此，该装置用于评估小鼠成纤维细胞（NIH 3T3）和人骨肉瘤细胞（SaOS2）在经各种细胞外基质蛋白功能化的表面上的迁移速率，以此证明将细胞迁移限制在微通道内可产生一致且可靠的数据。该装置设计能够在单个芯片上对多个重复样本进行快速且简单的评估，其中表面此前未接触过细胞或细胞分泌物。
