Galipienso Luis, Rubio Luis, López Luis, Soler Salvador, Aramburu José
Department of Plant Pathology, Institute of Agro-Alimentary and Technology Research (IRTA), 08348 Cabrils, Spain.
Virus Genes. 2009 Oct;39(2):256-60. doi: 10.1007/s11262-009-0388-4.
The genome of a Spanish isolate of Parietaria mottle virus (PMoV) obtained from tomato (strain PMoV-T) was completely sequenced. Protein motifs conserved for RNA viruses were identified: the p1 protein contained a metyltransferase domain in its N-terminal half and a triphosphatase/ helicase domain in its C-terminal half, the p2 protein contained a RNA polymerase domain; the 3a protein contained a RNA-binding domain with α-helix and β-sheet secondary structures. In addition, stem-loop structures with potential capacity of protein interactions were predicted on the untranslated terminal regions. Comparison with the other sequenced PMoV isolate showed nucleotide identities of 93, 90, and 93% for genomic RNAs 1, 2 and 3, respectively, and amino acid identities ranging from 88 to 97% for the different proteins. A cytosine deletion was detected at position 1,366 of RNA 3, involving a start codon for the coat protein (CP) gene different from the other PMoV isolate, resulting in a CP 16 amino acids shorter. Comparison of synonymous and nonsynonymous mutations revealed different selective constraints along the genome.
对从番茄中分离得到的西班牙墙草斑驳病毒（PMoV）毒株（PMoV-T）的基因组进行了全序列测定。鉴定出了RNA病毒保守的蛋白基序：p1蛋白在其N端一半含有一个甲基转移酶结构域，在其C端一半含有一个三磷酸酶/解旋酶结构域；p2蛋白含有一个RNA聚合酶结构域；3a蛋白含有一个具有α-螺旋和β-折叠二级结构的RNA结合结构域。此外，在非翻译末端区域预测到了具有潜在蛋白质相互作用能力的茎环结构。与其他已测序的PMoV分离株比较，基因组RNA 1、2和3的核苷酸同一性分别为93%、90%和93%，不同蛋白质的氨基酸同一性在88%至97%之间。在RNA 3的1366位检测到一个胞嘧啶缺失，涉及与其他PMoV分离株不同的外壳蛋白（CP）基因起始密码子，导致CP短16个氨基酸。同义突变和非同义突变的比较揭示了基因组不同区域的不同选择限制。
