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花生病毒C的全基因组序列,一种假定的新型等轴不稳环斑病毒。

Complete genome sequence of peanut virus C, a putative novel ilarvirus.

作者信息

Lim Seungmo, Jang Yun-Woo, Bae Jin Woo, Lee Yeong-Hoon, Lee Bong Choon, Yoon Youngnam

机构信息

Crop Production Technology Research Division, National Institute of Crop Science, Rural Development Administration, Miryang, 50424, Republic of Korea.

National Agricultural Products Quality Management Service, Busan, 46705, Republic of Korea.

出版信息

Arch Virol. 2018 Aug;163(8):2265-2269. doi: 10.1007/s00705-018-3827-5. Epub 2018 Apr 12.

Abstract

We determined the complete genome sequence of a putative novel ilarvirus, tentatively named "peanut virus C" (PVC), identified in peanut (Arachis hypogaea). The three segmented genomic RNA molecules of PVC were 3474 (RNA1), 2925 (RNA2), and 2160 (RNA3) nucleotides in length, with five predicted open reading frames containing conserved domains and motifs that are typical features of ilarviruses. The three genomic RNAs shared nucleotide sequence similarity (74% identity and 93% query coverage for RNA1, 75% identity and 85% query coverage for RNA2, and 72% identity and 70% query coverage for RNA3) with the most closely related ilarvirus, parietaria mottle virus. These results suggest that PVC is a novel member of the genus Ilarvirus in the family Bromoviridae.

摘要

我们测定了在花生(Arachis hypogaea)中鉴定出的一种假定的新型等轴不稳环斑病毒的全基因组序列,该病毒暂命名为“花生病毒C”(PVC)。PVC的三个分段基因组RNA分子长度分别为3474个核苷酸(RNA1)、2925个核苷酸(RNA2)和2160个核苷酸(RNA3),具有五个预测的开放阅读框,包含等轴不稳环斑病毒的典型特征——保守结构域和基序。这三个基因组RNA与亲缘关系最近的等轴不稳环斑病毒——墙草斑驳病毒,共享核苷酸序列相似性(RNA1的同一性为74%,查询覆盖率为93%;RNA2的同一性为75%,查询覆盖率为85%;RNA3的同一性为72%,查询覆盖率为70%)。这些结果表明,PVC是雀麦病毒科等轴不稳环斑病毒属的一个新成员。

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