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光合作用系统 II 的动力学模型应该考虑到供体侧猝灭对微秒时间范围内可变叶绿素 A 荧光的影响。

Kinetic models of photosystem II should accommodate the effect of donor side quenching on variable chlorophyll A fluorescence in the microseconds time range.

出版信息

Photosynth Res. 2009 Oct;102(1):99-101. doi: 10.1007/s11120-009-9477-4. Epub 2009 Jul 28.

DOI:10.1007/s11120-009-9477-4
PMID:19636807
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2755756/
Abstract

Quantitative data on laser flash-induced variable fluorescence in the 100 ns to 1 ms time range (Belyaeva et al. in Photosynth Res 98:105-119, 2008) confirming those of others (Steffen et al. in Biochemistry 40:173-180, 2001, Biochemistry 44:3123-3132, 2005; Belyaeva et al. in Biophysics 51(6):976-990, 2006), need a substantial correction with respect to magnitude of the normalized variable fluorescence associated with single turnover-induced charge separation in RCs of PS II. Their data are conclusive with the involvement of donor side quenching, the release of which occurs with a rate constant in the range of tens of ms(-1), and presumed to be associated with reduction of Y(+)(z) by the OEC.

摘要

Belyaeva 等人在 Photosynth Res 98:105-119, 2008 中报道了激光闪光在 100ns 至 1ms 时间范围内诱导的可变荧光的定量数据,证实了其他人的研究结果(Steffen 等人在 Biochemistry 40:173-180, 2001 和 Biochemistry 44:3123-3132, 2005 中的研究结果)。这些数据需要对与 PS II RC 中单 turnover 诱导的电荷分离相关的归一化可变荧光的幅度进行大量修正。他们的数据与供体侧猝灭的参与是一致的,供体侧猝灭的释放速率常数在几十毫秒(ms)(-1)范围内,据推测与 OEC 还原 Y(+)(z)有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/797b/2755756/f7c1c52acad6/11120_2009_9477_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/797b/2755756/f7c1c52acad6/11120_2009_9477_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/797b/2755756/f7c1c52acad6/11120_2009_9477_Fig1_HTML.jpg

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J Photochem Photobiol B. 2008 Apr 25;91(1):58-65. doi: 10.1016/j.jphotobiol.2008.01.005. Epub 2008 Jan 31.
3
On the chlorophyll a fluorescence yield in chloroplasts upon excitation with twin turnover flashes (TTF) and high frequency flash trains.关于叶绿体在双周转闪光(TTF)和高频闪光序列激发下的叶绿素a荧光产量。
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[The application of PS II model for the analysis of fluorescence yield transients induced by actinic single turnover flash in the time range from 100 ns to 10 s].[PS II模型在分析光化单次周转闪光在100纳秒至10秒时间范围内诱导的荧光产额瞬变中的应用]
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