Vredenberg Wim J
Department of Plant Physiology, Wageningen University and Research, Wageningen, Netherlands.
J Photochem Photobiol B. 2008 Apr 25;91(1):58-65. doi: 10.1016/j.jphotobiol.2008.01.005. Epub 2008 Jan 31.
The algorithm for simulation of the OJDIP fluorescence induction curve in chloroplasts under variable conditions is presented. It is derived from analyzes of chlorophyll a fluorescence kinetics upon excitation with single- (STF), twin- (TTF) and repetitive STF excitations, and from the rate equations that describe the sequence of transfer steps associated with the reduction of the primary quinone acceptor Q(A) and the release of photochemical fluorescence quenching of photosystem II (PSII) in multi-turnover excitation (MTF). The fluorescence induction algorithm (FIA) considers a photochemical O-J-D, a photo-electrochemical J-I and an I-P component (phase) which probably is associated with a photo-electric interaction between PSI and PSII. The photochemical phase incorporates the kinetics associated with the double reduction of the acceptor pair [PheQ(A)] in Q(B)-nonreducing reaction centers (RCs) and the associated doubling of the variable fluorescence, in agreement with the three-state trapping model (TSTM) of PSII. Application of and results with the algorithm are illustrated for MTF-induced OJDIP curves, measured in dark-adapted, in STF pre-excited and in DCMU inhibited thylakoids.
本文提出了一种在可变条件下模拟叶绿体中OJDIP荧光诱导曲线的算法。该算法源自对叶绿素a在单闪光（STF）、双闪光（TTF）和重复STF激发下的荧光动力学分析，以及描述多周转激发（MTF）中与初级醌受体Q(A)还原和光系统II（PSII）光化学荧光猝灭释放相关的转移步骤序列的速率方程。荧光诱导算法（FIA）考虑了一个光化学O-J-D、一个光电化学J-I和一个I-P成分（阶段），后者可能与PSI和PSII之间的光电相互作用有关。光化学阶段包含了与Q(B)非还原反应中心（RCs）中受体对[PheQ(A)]的双还原相关的动力学以及可变荧光的相应加倍，这与PSII的三态捕获模型（TSTM）一致。文中展示了该算法在MTF诱导的OJDIP曲线上的应用及结果，这些曲线是在暗适应、STF预激发和DCMU抑制的类囊体中测量得到的。
