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1H, 15N and 13C assignments of yellow fluorescent protein (YFP) Venus.

作者信息

Hsu Shang-Te Danny, Behrens Caroline, Cabrita Lisa D, Dobson Christopher M

机构信息

Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK.

出版信息

Biomol NMR Assign. 2009 Jun;3(1):67-72. doi: 10.1007/s12104-009-9143-y. Epub 2009 Jan 13.

DOI:10.1007/s12104-009-9143-y
PMID:19636949
Abstract

We present here the backbone and side-chain NMR assignments of YFP Venus, a 238-residue protein that emits yellow fluorescence in its native state. Venus is a variant of the green fluorescent protein (GFP), which has improved chromophore maturation and brightness, and the photochemistry and photophysics of which are insensitive to experimental conditions, such as the pH value and buffer content, making it a favourable biomarker.

摘要

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