34 kDa草履虫小球藻病毒1（PBCV1）DNA连接酶的序列特异性1H N、13C和15N主链共振归属
Sequence-specific 1H N, 13C, and 15N backbone resonance assignments of the 34 kDa Paramecium bursaria Chlorella virus 1 (PBCV1) DNA ligase.
作者信息
Piserchio Andrea, Nair Pravin A, Shuman Stewart, Ghose Ranajeet
机构信息
Department of Chemistry, The City College of New York, 160 Convent Avenue, New York, NY 10031, USA.
出版信息
Biomol NMR Assign. 2009 Jun;3(1):77-80. doi: 10.1007/s12104-009-9145-9. Epub 2009 Jan 13.
Abstract
Chlorella virus DNA ligase (ChVLig) is a minimal (298-amino acid) pluripotent ATP-dependent ligase composed of three structural modules--a nucleotidyltransferase domain, an OB domain, and a beta-hairpin latch--that forms a circumferential clamp around nicked DNA. ChVLig provides an instructive model to understand the chemical and conformational steps of nick repair. Here we report the assignment of backbone (13)C, (15)N, (1)H(N) resonances of this 34.2 kDa protein, the first for a DNA ligase in full-length form.
摘要
小球藻病毒DNA连接酶（ChVLig）是一种最小的（298个氨基酸）多能ATP依赖性连接酶，由三个结构模块组成——一个核苷酸转移酶结构域、一个OB结构域和一个β-发夹锁扣——它在缺口DNA周围形成一个圆周夹。ChVLig为理解缺口修复的化学和构象步骤提供了一个指导性模型。在这里，我们报告了这种34.2 kDa蛋白质的主链（13）C、（15）N、（1）H（N）共振归属，这是全长形式的DNA连接酶的首次报道。
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