牛白血病病毒在牛和绵羊分离的B淋巴细胞中的体外表达。

In vitro expression of bovine leukemia virus in isolated B-lymphocytes of cattle and sheep.

作者信息

Jensen W A, Sheehy S E, Fox M H, Davis W C, Cockerell G L

机构信息

Department of Pathology, Colorado State University, Fort Collins 80523.

出版信息

Vet Immunol Immunopathol. 1990 Dec;26(4):333-42. doi: 10.1016/0165-2427(90)90117-b.

DOI:10.1016/0165-2427(90)90117-b

PMID:1963713

Abstract

The purpose of this study was to determine the effect of T-lymphocytes and phytohemagglutinin (PHA), a T-cell mitogen, on the expression of bovine leukemia virus (BLV) in cultured B-lymphocytes from BLV-infected cattle and sheep. Bovine B-lymphocytes were isolated by negative selection via complement-mediated lysis of T-lymphocytes. Additionally, bovine and ovine B-lymphocytes were positively selected using fluorescence activated cell sorting. Expression of BLV in cultured bovine and ovine B-lymphocytes occurred in the absence of T-lymphocytes and without PHA stimulation. The results of this study demonstrate that BLV replication in cultured B-lymphocytes is T-cell independent. This finding may have implications for the mechanism of viral latency within infected B-lymphocytes.

摘要

本研究的目的是确定T淋巴细胞和T细胞有丝分裂原植物血凝素（PHA）对来自感染牛白血病病毒（BLV）的牛和绵羊的培养B淋巴细胞中BLV表达的影响。通过补体介导的T淋巴细胞裂解进行阴性选择来分离牛B淋巴细胞。此外，使用荧光激活细胞分选对牛和绵羊的B淋巴细胞进行阳性选择。在没有T淋巴细胞且无PHA刺激的情况下，培养的牛和绵羊B淋巴细胞中出现了BLV的表达。本研究结果表明，培养的B淋巴细胞中BLV的复制不依赖于T细胞。这一发现可能对感染的B淋巴细胞内病毒潜伏机制具有启示意义。