Department of Microbiology, Cornell University, Ithaca, NY 14853, USA.
Macromol Biosci. 2010 Feb 11;10(2):147-55. doi: 10.1002/mabi.200900175.
S-Layer proteins are an example of bionanostructures that can be exploited in nanofabrication. In addition to their ordered structure, the ability to self-assembly is a key feature that makes them a promising technological tool. Here, in vitro self-assembly kinetics of SpbA was investigated, and found that it occurs at a rate that is dependent on temperature, its concentration, and the concentration of calcium ions and sodium chloride. The activation enthalpy (120.81 kJ . mol(-1)) and entropy (129.34 J . mol(-1) . K(-1)) obtained infers that the incorporation of monomers incurs in a net loss of hydrophobic surface. By understanding how the protein monomers drive the self-assembly at different conditions, the rational optimization of this process was feasible.
S-层蛋白是一种可以在纳米制造中利用的生物纳米结构的例子。除了它们的有序结构外，自组装的能力是使它们成为有前途的技术工具的关键特征。在这里，研究了 SpbA 的体外自组装动力学，发现它的发生速率取决于温度、浓度以及钙离子和氯化钠的浓度。获得的活化焓（120.81 kJ·mol(-1)）和熵（129.34 J·mol(-1)·K(-1)）推断出单体的掺入导致疏水面的净损失。通过了解蛋白质单体如何在不同条件下驱动自组装，可以对该过程进行合理的优化。
