Iwama Y, Nakano T, Hasegawa K, Muto H
Department of Anatomy, Aichi Medical University, Nagakute, Japan.
Acta Anat (Basel). 1990;139(4):293-9. doi: 10.1159/000147013.
By means of immunohistochemistry, lactotropes, somatotropes, corticotropes and thyrotropes in the pituitary of the male musk shrew, Suncus murinus L., were identified at the electron-microscopic level. Lactotropes were classified into three types: type I containing large (200-450 nm in diameter) round secretory granules, type II with medium-sized (150-250 nm in diameter) ones and type III with small (50-150 nm in diameter) ones. Somatotropes were also classified into type I somatotropes that contain large (450 nm in diameter) spherical secretory granules and type II somatotropes containing comparatively small (300 nm in diameter) round granules. Both type I and II somatotropes were small and sometimes contained rod-shaped granules. Corticotropes were round or oval cells with round secretory granules in various densities and sizes (150-500 nm in diameter) scattered all over the cytoplasm. Thyrotropes were angular or polyhedral cells containing electron-transparent round secretory granules (200-300 nm in diameter) and large irregularly shaped granules with a maximum diameter of about 1,500 nm. Each type of the cells may be distinguished by its respective ultrastructural characteristics alone without the aid of immunohistochemistry.
通过免疫组织化学方法，在电子显微镜水平上鉴定了雄性麝鼩（Suncus murinus L.）垂体中的催乳激素细胞、生长激素细胞、促肾上腺皮质激素细胞和促甲状腺激素细胞。催乳激素细胞分为三种类型：I型含有大的（直径200 - 450纳米）圆形分泌颗粒，II型含有中等大小的（直径150 - 250纳米）颗粒，III型含有小的（直径50 - 150纳米）颗粒。生长激素细胞也分为I型生长激素细胞，其含有大的（直径450纳米）球形分泌颗粒，以及II型生长激素细胞，其含有相对小的（直径300纳米）圆形颗粒。I型和II型生长激素细胞都很小，有时还含有杆状颗粒。促肾上腺皮质激素细胞是圆形或椭圆形细胞，其圆形分泌颗粒大小和密度各异（直径150 - 500纳米），散布于整个细胞质中。促甲状腺激素细胞是角形或多面体细胞，含有电子透明的圆形分泌颗粒（直径200 - 300纳米）和最大直径约为1500纳米的大的不规则形状颗粒。仅通过各自的超微结构特征就可以区分每种类型的细胞，而无需借助免疫组织化学。
