Ishibashi T, Shiino M
Department of Anatomy, Wakayama Medical College, Japan.
Cell Tissue Res. 1988 Jan;251(1):111-6. doi: 10.1007/BF00215454.
Unique rod-shaped secretory granules were observed among oval or spherical secretory granules in GH cells of the anterior pituitary gland of musk shrew using the protein A-gold procedure combined with electron microscopy. The rod-shaped and spherical secretory granules were both immunoreactive by the immuno-gold method using antiserum to sheep GH. The rod-shaped secretory granules, which seem to be formed directly from the Golgi vesicles, extend from several hundred to several thousand nm in length. They often touch each other and fuse. The spherical secretory granules are also unique in that they may also fuse with loss of dense contents to leave "empty" circular membrane profiles in the cytoplasm. Both the rod-shaped and spherical secretory granules are secreted from the cell by exocytosis.
运用蛋白A-金标法结合电子显微镜技术，在麝鼩垂体前叶生长激素（GH）细胞的椭圆形或球形分泌颗粒中观察到了独特的杆状分泌颗粒。采用抗绵羊生长激素抗血清的免疫金标法显示，杆状和球形分泌颗粒均具有免疫反应性。杆状分泌颗粒似乎直接由高尔基体囊泡形成，长度从几百纳米到几千纳米不等。它们常常相互接触并融合。球形分泌颗粒的独特之处还在于，它们也可能融合，同时致密内容物丢失，在细胞质中留下“空的”圆形膜轮廓。杆状和球形分泌颗粒均通过胞吐作用从细胞中分泌出来。
