[从长柄樟（N. Chao ex H. W. Li）中分离的内生芽孢杆菌的系统发育多样性]
[Phylogenetic diversity of endophytic endospore-forming bacteria isolated from Cinnamomum longepaniculatum N. Chao ex H. W. Li].
作者信息
Wang Tao, You Ling, Cui Xiaolong, Tian Wen, Huang Naiyao, Li Xinglong
机构信息
Key Laboratory of Fermentation Resources and Application at Universities of Sichuan Province, Yibin University, Yibin 644000, China.
出版信息
Wei Sheng Wu Xue Bao. 2009 May;49(5):573-9.
OBJECTIVE
In order to study the diversity of endophytic endospore-forming bacteria in Cinnamomum longepaniculatum.
METHODS
We took modified nutrient agar medium for isolation and cultivation and analyzed the 16S rRNA gene sequences of the isolates.
RESULTS
Forty non-redundant endospore-forming bacterial isolates were ascertained, which accounted for 38.1% of all the endophytic bacterial isolates. Of them, 24 isolates were from roots, 7 from stems and 9 from leaves. Phylogenetic analysis based on 16S rRNA gene sequences showed that 35 of them belonged to 16 species of the genera Bacillus, Lysinibacillus and Paenibacillus, and 5 isolates with < 97% sequence similarities to their closely related members were presumed to be potential novel species.
CONCLUSION
The results showed that the cultivable endospore-forming bacteria diversity was abundant and there were some potential novel strains in Cinnamomum longepaniculatum. The microflora of endophytic endospore-forming bacteria in individuals of C. longepaniculatum showed that some bacteria distributed in different organs, but the others were organ-specific bacteria.
目的
研究长柄樟内生芽孢杆菌的多样性。
方法
采用改良营养琼脂培养基进行分离培养，并对分离菌株的16S rRNA基因序列进行分析。
结果
确定了40株非冗余芽孢杆菌分离株，占所有内生细菌分离株的38.1%。其中，24株分离自根，7株分离自茎，9株分离自叶。基于16S rRNA基因序列的系统发育分析表明，其中35株属于芽孢杆菌属、赖氨酸芽孢杆菌属和类芽孢杆菌属的16个种，5株与亲缘关系最近的成员序列相似性小于97%的分离株被推测为潜在新种。
结论
结果表明，长柄樟中可培养的芽孢杆菌多样性丰富，存在一些潜在新菌株。长柄樟个体内生芽孢杆菌的微生物群落表明，一些细菌分布于不同器官，而另一些则是器官特异性细菌。
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