Zhou Fu-cai, Zhou Gui-sheng, Li Chuan-ming, Yang Yi-zhong, Qin Pei
School of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, Jiangsu, China.
Ying Yong Sheng Tai Xue Bao. 2009 Mar;20(3):603-8.
To test the feasibility of using raw extracts from the tissues of biomass energy plants Ricinus communi and Kosteletzkya virginica as plant protection agents, the alcohol extracts from R. communi seed and leaf and from K. virginica leaf were used to treat adult Bemisia tabaci by spraying. The glutathione S-transferase and carboxylesterase activities in B. tabaci body were measured after treated for 4 h, 24 h, 48 h, 72 h, and 96 h, and the olfaction responses of B. tabaci to the alcohol extracts were detected with a Y-tube olfactomet. All the three alcohol extracts obviously inhibited the glutathione S-transferase and carboxylesterase activities in a concentration-dependent manner. The inhibitory effect of the 250-times diluted alcohol extracts on the two enzyme activities was equivalent to that of 3000 times-diluted 1.8% avermectins. In addition, the 250-times diluted alcohol extracts had obvious repellent effect on B. tabaci, with the repellent coefficient of the alcohol extracts from R. communi seed and leaf and from K, virginica leaf being 100.0%, 96.7%, and 79.4%, respectively. All of these suggested that the test three alcohol extracts had repellent and other biological effects on B. tabaci.
为测试将生物质能源植物蓖麻和弗吉尼亚盐角草的组织粗提物用作植物保护剂的可行性，采用蓖麻种子和叶片以及弗吉尼亚盐角草叶片的乙醇提取物通过喷雾处理烟粉虱成虫。在处理4小时、24小时、48小时、72小时和96小时后，测定烟粉虱体内谷胱甘肽S-转移酶和羧酸酯酶的活性，并用Y型嗅觉仪检测烟粉虱对乙醇提取物的嗅觉反应。所有三种乙醇提取物均以浓度依赖的方式显著抑制谷胱甘肽S-转移酶和羧酸酯酶的活性。250倍稀释的乙醇提取物对这两种酶活性的抑制作用与3000倍稀释的1.8%阿维菌素相当。此外，250倍稀释的乙醇提取物对烟粉虱有明显的驱避作用，蓖麻种子和叶片以及弗吉尼亚盐角草叶片的乙醇提取物的驱避系数分别为100.0%、96.7%和79.4%。所有这些表明，所测试的三种乙醇提取物对烟粉虱具有驱避和其他生物学作用。
