Orchard G E, Torres J, Poirier A, Sounthararajah R, Webster J, Notini L, Hacker L, Ismail F, Nwokie T, Humphrey P, Spigler E, Missaghian-Cully S, Brewer C, Meredith-Jones A
St. John's Histopathology Department, St. Thomas' Hospital, London SE 7EH, UK.
Br J Biomed Sci. 2009;66(2):63-6. doi: 10.1080/09674845.2009.11730246.
The use of tissue softening agents to improve microtomy of keratotic tissues is employed widely. Many of these softeners contain hazardous constituents such as phenol. In this study, the use of non-ionic surfactants or non-toxic ingredients are investigated with the aim of creating a new softening agent. The new agent should be more effective in facilitating the sectioning of hardened tissue while reducing toxicity and complications associated with sectioning hard tissue compared to a commercially available phenol-based formulation. Four formulations are compared against the commercial product for their capability to section routinely processed paraffin-embedded tissue under standard operating procedure parameters. The trial formulations were shown to be fast acting and enabled improved serial sectioning of hard keratotic tissue in nearly all the cases tested. There was no evidence of adverse staining using either tinctorial or immunohistochemical methods. The new formulations had advantages over the commercially available solutions, improving on the number and quality of sections attainable from the tissue blocks, as well as offering a composition less toxic than phenol-based products.
使用组织软化剂来改善角化组织的切片技术已被广泛应用。这些软化剂中的许多都含有诸如苯酚等有害成分。在本研究中，对使用非离子表面活性剂或无毒成分进行了调查，目的是开发一种新型软化剂。与市售的基于苯酚的制剂相比，新型制剂应在促进硬化组织切片方面更有效，同时减少与硬组织切片相关的毒性和并发症。在标准操作程序参数下，将四种制剂与市售产品进行比较，以评估它们对常规处理的石蜡包埋组织进行切片的能力。试验制剂显示起效迅速，在几乎所有测试案例中都能改善硬角化组织的连续切片。无论是采用染色法还是免疫组织化学方法，均未发现不良染色的证据。新制剂比市售溶液具有优势，能提高从组织块获得的切片数量和质量，并且其成分的毒性低于基于苯酚的产品。
