切片术：切割福尔马林固定、石蜡包埋切片
Microtomy: Cutting Formalin-Fixed, Paraffin-Embedded Sections.
作者信息
Sy Joanne, Ang Lee-Cyn
机构信息
Department of Anatomical Pathology, Concord Repatriation General Hospital, University of Sydney, Concord, NSW, Australia.
Department of Pathology (Neuropathology) and Laboratory Medicine, University Hospital - London Health Sciences Centre, Western University, Ontario, London, ON, Canada.
出版信息
Methods Mol Biol. 2019;1897:269-278. doi: 10.1007/978-1-4939-8935-5_23.
Formalin-fixed, paraffin-embedded tissue (FFPE) still plays an important role in biobanking, since it is comparatively easy to obtain and store in comparison to fresh frozen tissue. They are stored as paraffin or FFPE blocks. Unstained slides derived from FFPE blocks may be used for hematoxylin and eosin histology, special stains, immunohistochemistry, and chromogenic or fluorescent in situ hybridization. In addition, tissue scraped off FFPE slides or from scrolls of FFPE tissue may be used for molecular or proteomic analyses. Hematoxylin and eosin staining of FFPE sections reviewed by a pathologist are highly valuable to ensure the presence of adequate lesional cells for molecular and other analyses. Therefore, proper microtomy technique is essential in the preparation of formalin-fixed, paraffin-embedded tissue for biobanking purposes. Here we describe the process of cutting paraffin embedded sections using a rotary microtome. We also highlight the possible pitfalls that may arise and discuss how to avoid them.
福尔马林固定石蜡包埋组织（FFPE）在生物样本库中仍发挥着重要作用，因为与新鲜冷冻组织相比，它相对容易获取和储存。它们以石蜡块或FFPE块的形式储存。源自FFPE块的未染色玻片可用于苏木精和伊红组织学、特殊染色、免疫组织化学以及显色或荧光原位杂交。此外，从FFPE玻片上刮下的组织或FFPE组织切片条可用于分子或蛋白质组学分析。病理学家对FFPE切片进行苏木精和伊红染色对于确保存在足够的病变细胞用于分子和其他分析非常有价值。因此，在为生物样本库目的制备福尔马林固定石蜡包埋组织时，适当的切片技术至关重要。在这里，我们描述了使用旋转切片机切割石蜡包埋切片的过程。我们还强调了可能出现的陷阱并讨论了如何避免它们。
Zotero 插件