National Research Centre on Pig, Indian Council of Agricultural Research, Rani, Guwahati, India.
Zoonoses Public Health. 2010 Sep;57(6):397-401. doi: 10.1111/j.1863-2378.2009.01249.x.
Faecal samples obtained from 190 healthy mithuns were examined for the presence of Escherichia coli. Total one-hundred and five E. coli isolates were obtained from these samples, which belonged to 55 different serogroups. These isolates were subjected to multiplex polymerase chain reaction (m-PCR) for detection of stx1, stx2, eaeA and hlyA genes. Twenty-three (21.90%) E. coli isolates belonging to 14 serogroups revealed the presence of at least one virulence gene when examined by m-PCR. Nineteen percent and 2.85% of the mithuns were found to carry Shiga toxin-producing E. coli (STEC) and enteropathogenic E. coli, respectively. stx1 and stx2 genes were found to be prevalent in 7 (6.67%) and 18 (17.14%) of the isolates respectively, whereas eaeA and hlyA genes were found to be carried by three (2.85% each) isolates. Interestingly, none of the STEC isolates belonged to serogroup O157.
从 190 头健康的野牛中采集粪便样本，检测是否存在大肠杆菌。从这些样本中总共获得了 105 株大肠杆菌分离株，它们属于 55 个不同的血清群。这些分离株通过多重聚合酶链反应（m-PCR）检测stx1、stx2、eaeA 和 hlyA 基因。通过 m-PCR 检测，属于 14 个血清群的 23 株（21.90%）大肠杆菌分离株至少携带一个毒力基因。19%和 2.85%的野牛被发现携带产志贺毒素大肠杆菌（STEC）和肠致病性大肠杆菌。stx1 和 stx2 基因分别在 7 株（6.67%）和 18 株（17.14%）分离株中普遍存在，而 eaeA 和 hlyA 基因分别由 3 株（2.85%）分离株携带。有趣的是，没有一株 STEC 分离株属于 O157 血清群。
