Aquadro C F, Tachida H, Langley C H, Harada K, Mukai T
Laboratory of Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
Genetics. 1990 Dec;126(4):915-9. doi: 10.1093/genetics/126.4.915.
We present here a molecular analysis of the region surrounding the structural gene encoding alcohol dehydrogenase (Adh) in 47 lines of Drosophila melanogaster that have each accumulated mutations for 300 generations. While these lines show a significant increase in variation of alcohol dehydrogenase enzyme activity compared to control lines, we found no restriction map variation in a 13-kb region including the complete Adh structural gene and roughly 5 kb of both 5' and 3' sequences. Thus, the rapid accumulation of ADH activity variation after 28,200 allele generations does not appear to have been due to the mobilization of transposable elements into or out of the Adh structural gene region.
我们在此展示了对47条黑腹果蝇品系中编码乙醇脱氢酶（Adh）的结构基因周围区域的分子分析，这些品系每条都经历了300代的突变积累。虽然与对照品系相比，这些品系的乙醇脱氢酶活性变异显著增加，但我们发现在一个13kb的区域内没有限制性图谱变异，该区域包括完整的Adh结构基因以及大约5kb的5'和3'序列。因此，在28200个等位基因世代后ADH活性变异的快速积累似乎并非由于转座元件向Adh结构基因区域内或从该区域转出所致。
