Hu Honglin, Wang Gongxian, Batteux Frédéric, Nicco Carole
Department of Surgery, The Medical College of Nanchang University, No. 17 Yongwai Street, Nanchang, P.R. China.
Tohoku J Exp Med. 2009 Aug;218(4):325-9. doi: 10.1620/tjem.218.325.
Recent studies have shown the remarkable gender differences in the susceptibility or expression of many diseases. The mechanism underlying the gender differences is unclear. In the present study, we evaluated the effects of gender differences and different ischemia time on the renal ischemia-reperfusion injury (IRI). The IRI was induced in the bilateral kidneys of 156 male and 30 female BALB/c mice. Renal function, serum creatinine and blood urea nitrogen, and pathology of the kidneys were examined at 24 hr after IRI. Renal IRI was generated successfully in 182 of 186 mice with a 97.85% success rate. The levels of serum creatinine and blood urea nitrogen were significantly increased in male mice subjected to 30 min, 35 min, or 45 min of renal ischemia and in female mice subjected to 75 min of renal ischemia, compared to the control group at 24 hr after operation. In males following 35 min or 45 min of ischemia and in females following 75 min of ischemia, typical acute tubular necrosis was found in the areas of corticomedullary junction and the histopathologic scores, which represent the degree of renal tissue injuries, were significantly increased. In view of our data, the kidneys of male are much more susceptible to IRI than those of female. The optimal ischemia time of kidney is 35-45 min in males and 75 min in females for generating a stable model of IRI in mice. Investigation of the gender differences might provide a new area for mechanistic study of renal IRI.
最近的研究表明，在许多疾病的易感性或表现方面存在显著的性别差异。性别差异背后的机制尚不清楚。在本研究中，我们评估了性别差异和不同缺血时间对肾缺血再灌注损伤（IRI）的影响。在156只雄性和30只雌性BALB/c小鼠的双侧肾脏中诱导IRI。在IRI后24小时检查肾功能、血清肌酐和血尿素氮以及肾脏病理。186只小鼠中有182只成功产生了肾IRI，成功率为97.85%。与术后24小时的对照组相比，经历30分钟、35分钟或45分钟肾缺血的雄性小鼠以及经历75分钟肾缺血的雌性小鼠的血清肌酐和血尿素氮水平显著升高。在缺血35分钟或45分钟后的雄性小鼠以及缺血75分钟后的雌性小鼠中，在皮质髓质交界处发现典型的急性肾小管坏死，并且代表肾组织损伤程度的组织病理学评分显著增加。根据我们的数据，雄性小鼠的肾脏比雌性小鼠更容易受到IRI的影响。在小鼠中建立稳定的IRI模型时，雄性肾脏的最佳缺血时间为35 - 45分钟，雌性为75分钟。对性别差异的研究可能为肾IRI的机制研究提供一个新的领域。
