Kianian Farzaneh, Seifi Behjat, Kadkhodaee Mehri, Sajedizadeh Abdullah, Ahghari Parisa
Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Department of Physiology, School of Medicine, Hamedan University of Medical Sciences, Hamedan, Iran.
Iran J Basic Med Sci. 2019 Jan;22(1):43-48. doi: 10.22038/ijbms.2018.29644.7156.
There is increasing evidence for the importance of gender in different diseases; however, the role of gender in response to treatments is still unknown. Therefore, this study investigated the impact of gender on the protective effects of celecoxib in ischemia reperfusion (IR)-induced acute kidney injury.
In this experimental study, rats were randomly divided into 6 groups (n=6): IR, sham and celecoxib groups of males and females. In IR groups, after orally receiving saline for 5 days, renal pedicles were clamped for 55 min and then kidneys were reperfused for 24 hr. In the sham groups, clamping of renal pedicles was not performed. In the celecoxib groups, 30 mg/kg celecoxib was given orally for 5 days before induction of ischemia. Plasma was collected to determine creatinine (Cr) and blood urea nitrogen (BUN). Kidney tissue samples were also stored for examining the histopathology and measuring malondialdehyde (MDA) levels and superoxide dismutase (SOD) activities.
IR caused significant increases in plasma Cr (0.05), BUN (0.05) and renal histopathological damages in both genders. Also, induction of IR resulted in significant increase of MDA levels (0.05) and decrease of SOD activities (0.05) in the kidney in both genders. Celecoxib administration prevented the IR-induced functional, histopathological and oxidative changes in both genders by similar degrees.
This study suggested that in similar pathological conditions, celecoxib improves renal function and histopathological damages and attenuates oxidative stress in both genders by the same degrees. These protective effects of celecoxib on IR-induced kidney injury are gender-independent.
越来越多的证据表明性别在不同疾病中具有重要性；然而，性别在治疗反应中的作用仍不明确。因此，本研究调查了性别对塞来昔布在缺血再灌注（IR）诱导的急性肾损伤中的保护作用的影响。
在本实验研究中，大鼠被随机分为6组（n = 6）：雄性和雌性的IR组、假手术组和塞来昔布组。在IR组中，大鼠口服生理盐水5天后，肾蒂夹闭55分钟，然后肾脏再灌注24小时。在假手术组中，不进行肾蒂夹闭。在塞来昔布组中，在诱导缺血前5天口服给予30 mg/kg塞来昔布，持续5天。收集血浆以测定肌酐（Cr）和血尿素氮（BUN）。还保存肾组织样本以检查组织病理学并测量丙二醛（MDA）水平和超氧化物歧化酶（SOD）活性。
IR导致两性血浆Cr（P < 0.05）、BUN（P < 0.05）显著升高以及肾脏组织病理学损伤。此外，IR诱导导致两性肾脏中MDA水平显著升高（P < 0.05）和SOD活性降低（P < 0.05）。给予塞来昔布可在相似程度上预防IR诱导的两性功能、组织病理学和氧化变化。
本研究表明，在相似的病理条件下，塞来昔布可同等程度地改善两性的肾功能和组织病理学损伤，并减轻氧化应激。塞来昔布对IR诱导的肾损伤的这些保护作用与性别无关。
