Lai Xianyin, Blazer-Yost Bonnie L, Gattone Vincent H, Muchatuta Monalisa N, Witzmann Frank A
Department of Cellular & Integrative Physiology, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
Proteomics. 2009 Jul;9(14):3775-82. doi: 10.1002/pmic.200800379.
Cysts arising from hepatic bile ducts are a common extra-renal pathology associated with polycystic kidney disease in humans. As an initial step in identifying active components that could contribute to disease progression, we have investigated the protein composition of hepatic cyst fluid in an orthologous animal model of autosomal recessive polycystic kidney disease, heterozygous (BALB/c-cpk/+) mice. Proteomic analysis of cyst fluid tryptic digests using LC-MS/MS identified 303 proteins, many of which are consistent with enhanced inflammatory cell processes, cellular proliferation, and basal laminar fibrosis associated with the development of hepatic bile duct cysts. Protein identifications have been submitted to the PRIDE database (http://www.ebi.ac.uk/pride), accession number 9227.
源自肝内胆管的囊肿是人类多囊肾病常见的肾外病理表现。作为确定可能导致疾病进展的活性成分的第一步，我们在常染色体隐性多囊肾病的直系同源动物模型——杂合子（BALB/c-cpk/+）小鼠中，研究了肝囊肿液的蛋白质组成。使用LC-MS/MS对囊肿液胰蛋白酶消化产物进行蛋白质组学分析，鉴定出303种蛋白质，其中许多与肝内胆管囊肿发展相关的炎症细胞过程增强、细胞增殖和基底膜纤维化一致。蛋白质鉴定结果已提交至PRIDE数据库（http://www.ebi.ac.uk/pride），登录号为9227。
