Wang Yilin, Ding Chunguang, Du Kehe, Xiao Yao, Wu Caisheng, Zhang Jinlan, Qin Hailin, Du Guanhua
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
Rapid Commun Mass Spectrom. 2009 Sep;23(17):2724-32. doi: 10.1002/rcm.4179.
Xiao-xu-ming decoction (XXMD) prescription is a traditional Chinese prescription that has been widely used to treat theoplegia and the sequela of theoplegia. Modern pharmacological research has also indicated that the active fraction from XXMD is able to treat cardiovascular diseases and Alzheimer's disease. In the study reported here, high-performance liquid chromatography coupled with Fourier transform ion cyclotron resonance mass spectrometry (HPLC/FTICR-MS) was developed to identify active compounds and their metabolites after oral administration of active fraction from Xiao-xu-ming decoction to rats, using parent mass list triggered data-dependent multiple-stage mass analysis at a resolving power of 100,000 in the external calibration mode. The mass accuracies obtained for full-scan MS were within 2 ppm in most cases. Fifteen constituents were identified in the active fraction from XXMD and the biological samples of rats. The fragmentation behaviors of these constituents were summarized which would be helpful for structural characterization. The profiles of the constituents in the active fraction and biological samples of rats were obtained which provided us with much information for a better understanding of the chemical basis of the pharmacologic actions of XXMD.
小续命汤（XXMD）方剂是一种传统的中药方剂，已被广泛用于治疗中风及中风后遗症。现代药理学研究也表明，小续命汤的活性成分能够治疗心血管疾病和阿尔茨海默病。在本文报道的研究中，开发了高效液相色谱-傅里叶变换离子回旋共振质谱联用技术（HPLC/FTICR-MS），以鉴定小续命汤活性成分口服给予大鼠后其活性化合物及其代谢产物，在外标模式下采用母离子质量列表触发的数据依赖型多级质谱分析，分辨率为100,000。在大多数情况下，全扫描质谱获得的质量准确度在2 ppm以内。从小续命汤活性成分及大鼠生物样品中鉴定出15种成分。总结了这些成分的裂解行为，这将有助于结构表征。获得了小续命汤活性成分及大鼠生物样品中成分的图谱，为更好地理解小续命汤药理作用的化学基础提供了很多信息。
