Dooper M M B W, Plassen C, Holden L, Lindvik H, Faeste C K
National Veterinary Institute, Oslo, Norway.
J Investig Allergol Clin Immunol. 2009;19(4):283-91.
Lupine is used increasingly in food products. The development of lupine allergy in peanut-allergic patients is believed to occur as a result of cross-reactivity between lupine and peanut proteins.
To investigate the degree of immunoglobulin (Ig) E cross-reactivity between allergens in lupine and peanut.
We investigated IgE cross-reactivity between lupine alpha-, beta-, gamma-, and delta-conglutins and the major peanut allergens Ara h 1, Ara h 2 and Ara h 3 using enzyme-linked immunosorbent assay with sera from patients with coexisting peanut and lupine allergy.
Peanut proteins inhibited IgE binding towards alpha- conglutins, delta-conglutins, and, to a lesser degree, beta-conglutins, while no IgE cross-reaction with delta-conglutin was observed. Ara h 2 most potently inhibited IgE binding to lupine and delta-conglutins, while Ara h 1 most potently cross-reacted with beta-conglutin. Ara h 3 was apparently not involved in these mechanisms.
The present study reveals IgE cross-reactivity between the 2S albumins Ara h 2 and delta-conglutin, and the 7S vicilin-like Ara h 1 and beta-conglutin, which are possibly based on homologies between phylogenetically related proteins. Ara h 2 was the most potent inhibitor of IgE binding to lupine conglutins.
羽扇豆在食品中的使用日益增加。花生过敏患者中羽扇豆过敏的发生被认为是羽扇豆和花生蛋白之间交叉反应的结果。
研究羽扇豆和花生中过敏原之间免疫球蛋白（Ig）E交叉反应的程度。
我们使用酶联免疫吸附测定法，以同时对花生和羽扇豆过敏患者的血清，研究羽扇豆α-、β-、γ-和δ-伴球蛋白与主要花生过敏原Ara h 1、Ara h 2和Ara h 3之间的IgE交叉反应。
花生蛋白抑制IgE与α-伴球蛋白、δ-伴球蛋白以及程度较轻的β-伴球蛋白的结合，而未观察到与δ-伴球蛋白的IgE交叉反应。Ara h 2最有效地抑制IgE与羽扇豆和δ-伴球蛋白的结合，而Ara h 1与β-伴球蛋白的交叉反应最为明显。Ara h 3显然未参与这些机制。
本研究揭示了2S清蛋白Ara h 2和δ-伴球蛋白以及7S类豌豆球蛋白Ara h 1和β-伴球蛋白之间的IgE交叉反应，这可能基于系统发育相关蛋白之间的同源性。Ara h 2是IgE与羽扇豆伴球蛋白结合的最有效抑制剂。
