Tahmasian Arineh, Juhász Angéla, Broadbent James A, Nye-Wood Mitchell G, Le Thao T, Colgrave Michelle L
Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science, School of Science, Edith Cowan University, Joondalup, WA, Australia.
Commonwealth Scientific and Industrial Research Organisation Agriculture and Food, St. Lucia, QLD, Australia.
Front Nutr. 2022 May 13;9:842168. doi: 10.3389/fnut.2022.842168. eCollection 2022.
Lupin seeds have an excellent nutritional profile, including a high proportion of protein and dietary fiber. These qualities make lupin seeds an ideal candidate to help meet the growing global demand for complementary sources of protein. Of consequence to this application, there are nutritional and antinutritional properties assigned to the major lupin seed storage proteins-referred to as α-, β-, δ- and γ-conglutins The variation in the abundance of these protein families can impact the nutritional and bioactive properties of different lupin varieties. Hence, exploring the conglutin protein profiles across a diverse range of lupin varieties will yield knowledge that can facilitate the selection of superior genotypes for food applications or lupin crop improvement. To support this knowledge generation, discovery proteomics was applied for the identification of the 16 known conglutin subfamilies from 46 domestic and wild narrow-leafed lupin (NLL) genotypes. Consequently, the diversity of abundance of these proteins was evaluated using liquid chromatography-multiple reaction monitoring-mass spectrometry (LC-MRM-MS). This comparative study revealed a larger variability for the β- and δ-conglutin content across the lines under study. The absence/lower abundance of the β2- to β6-conglutin subfamilies in a subset of the domesticated cultivars led to substantially lower overall levels of the allergenic β-conglutin content in these NLLs, for which the elevation of the other conglutin families were observed. The diversity of the conglutin profiles revealed through this study-and the identification of potential hypoallergenic genotypes-will have great significance for lupin allergic consumers, food manufactures as well as grain breeders through the future development of lupin varieties with higher levels of desirable bioactive proteins and lower allergen content.
羽扇豆种子具有出色的营养成分，包括高比例的蛋白质和膳食纤维。这些特性使羽扇豆种子成为满足全球对补充蛋白质来源不断增长需求的理想选择。对于这种应用而言，主要的羽扇豆种子贮藏蛋白（称为α-、β-、δ-和γ-凝集素）具有营养和抗营养特性。这些蛋白质家族丰度的变化会影响不同羽扇豆品种的营养和生物活性特性。因此，探索多种羽扇豆品种的凝集素蛋白谱将产生有助于选择用于食品应用的优良基因型或改良羽扇豆作物的知识。为了支持这一知识生成，应用发现蛋白质组学从46个国内和野生窄叶羽扇豆（NLL）基因型中鉴定出16个已知的凝集素亚家族。随后，使用液相色谱-多反应监测-质谱（LC-MRM-MS）评估这些蛋白质丰度的多样性。这项比较研究揭示了在所研究的品系中，β-和δ-凝集素含量的变异性更大。在一部分驯化品种中，β2至β6凝集素亚家族的缺失/低丰度导致这些NLL中致敏性β-凝集素的总体含量大幅降低，同时观察到其他凝集素家族含量升高。通过这项研究揭示的凝集素谱的多样性以及潜在低致敏基因型的鉴定，对于羽扇豆过敏消费者、食品制造商以及谷物育种者在未来开发具有更高水平理想生物活性蛋白和更低过敏原含量的羽扇豆品种方面具有重要意义。
