Department of Animal Biotechnology, College of Animal Bioscience & Technology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
Virol J. 2009 Jul 30;6:115. doi: 10.1186/1743-422X-6-115.
As noted in other flaviviruses, the envelope (E) protein of Japanese encephalitis virus (JEV) interacts with a cellular receptor and mediates membrane fusion to allow viral entry into target cells, thus eliciting neutralizing antibody response. The formation of the flavivirus prM/E complex is followed by the cleavage of precursor membrane (prM) and membrane (M) protein by a cellular signalase. To test the effect of prM in JEV biology, we constructed JEV-MuLV pseudotyped viruses that express the prM/E protein or E only. The infectivity and titers of JEV pseudotyped viruses were examined in several cell lines. We also analyzed the neutralizing capacities with anti-JEV sera from JEV-immunized mice. Even though prM is crucial for multiple stages of JEV biology, the JEV-pseudotyped viruses produced with prM/E or with E only showed similar infectivity and titers in several cell lines and similar neutralizing sensitivity. These results showed that JEV-MuLV pseudotyped viruses did not require prM for production of infectious pseudotyped viruses.
如其他黄病毒所述，日本脑炎病毒（JEV）的包膜（E）蛋白与细胞受体相互作用，并介导膜融合以允许病毒进入靶细胞，从而引发中和抗体反应。黄病毒前膜（prM）和膜（M）蛋白的形成随后被细胞信号酶切割。为了测试 prM 在 JEV 生物学中的作用，我们构建了表达 prM/E 蛋白或仅 E 蛋白的 JEV-MuLV 假型病毒。我们在几种细胞系中检测了 JEV 假型病毒的感染性和滴度。我们还使用来自 JEV 免疫小鼠的抗 JEV 血清分析了中和能力。尽管 prM 对 JEV 生物学的多个阶段至关重要，但用 prM/E 或仅用 E 产生的 JEV 假型病毒在几种细胞系中显示出相似的感染性和滴度，并且具有相似的中和敏感性。这些结果表明，JEV-MuLV 假型病毒不需要 prM 来产生感染性假型病毒。
