Herrera-Vásquez José Angel, Cebrián María Del Carmen, Alfaro-Fernández Ana, Córdoba-Sellés María Del Carmen, Jordá Concepción
Grupo de Virología, Instituto Agroforestal Mediterráneo, Universidad Politécnica de Valencia, Camino de Vera s/n, E-46022 Valencia, Spain.
Mycol Res. 2009 May;113(5):602-10. doi: 10.1016/j.mycres.2009.01.007. Epub 2009 Feb 7.
A multiplex PCR method has been developed to detect, differentiate, and confirm the morphological identification of three root infecting Olpidium spp.: O. bornovanus, O. brassicae, and O. virulentus. Of the 132 root samples examined, 101 samples were infected by Olpidium spp.. Based on the morphology of resting spores, the presence of O. bornovanus was confirmed in 20.5% of the samples, whereas species identity could not be determined for the remaining samples because they failed to reproduce sexually. With multiplex PCR, it was possible to determine the Olpidium identity of all the infected samples, even when resting spores were not formed. This method was also effective for detecting Olpidium spp. in water samples. In addition, the specificity and sensitivity of multiplex PCR were evaluated. The multiplex PCR method was validated with samples of 9 different crops from 11 countries of America, Europe, and Africa.
已开发出一种多重PCR方法，用于检测、区分和确认三种侵染根部的油壶菌属物种：博诺瓦油壶菌、芸苔油壶菌和致病油壶菌的形态学鉴定。在检测的132个根样本中，有101个样本被油壶菌属感染。根据休眠孢子的形态，在20.5%的样本中确认存在博诺瓦油壶菌，而其余样本由于未能进行有性繁殖，无法确定其物种身份。使用多重PCR，即使未形成休眠孢子，也能够确定所有感染样本的油壶菌身份。该方法对于检测水样中的油壶菌属也有效。此外，还评估了多重PCR的特异性和灵敏度。多重PCR方法已通过来自美洲、欧洲和非洲11个国家的9种不同作物的样本进行了验证。
