Sersen F, Balgavý P, Devínsky F
Faculty of Sciences, J. A. Comenius University.
Gen Physiol Biophys. 1990 Dec;9(6):625-33.
Electron spin resonance spectroscopy (ESR) was used to study the effects of amphiphilic amines of the carbamate, amide, and ester type and amine oxide on the photosynthetic system of spinach chloroplasts. The ESR signal II connected to the photosynthetic center PS II donor side was observed to diminish in the presence of amines, whereas that of PS I remained unchanged. The inhibition of PS II increased with the increasing of amine concentration. In the presence of amines, the light: dark chloroplast ESR signals ratio as well as the intensity of the ESR signal of unbound Mn2+ increased. It is suggested that the amphiphilic amines affect the structure of PS II and the electron transfer to PS I. The effects of the amines tested on the photosynthetic system correlate with their potency to perturb the lipid membrane structure.
采用电子自旋共振光谱（ESR）研究了氨基甲酸酯型、酰胺型和酯型两亲性胺以及氧化胺对菠菜叶绿体光合系统的影响。观察到，在胺存在的情况下，与光合中心PS II供体侧相关的ESR信号II减弱，而PS I的信号保持不变。PS II的抑制作用随胺浓度的增加而增强。在胺存在的情况下，光：暗叶绿体ESR信号比率以及未结合Mn2+的ESR信号强度增加。表明两亲性胺会影响PS II的结构以及向PS I的电子转移。所测试的胺对光合系统的影响与其扰乱脂质膜结构的能力相关。
