School of Chemical Engineering and Technology, Tianjin University, Nankai District, Tianjin, People's Republic of China.
J Mater Sci Mater Med. 2010 Jan;21(1):319-27. doi: 10.1007/s10856-009-3833-y. Epub 2009 Jul 30.
Hepatocyte-specific three-dimensional tissue-engineeringed scaffold plays an important role for developing bioartificial liver devices. In the present study, galactose moieties were covalently coupled with hyaluronic acid through ethylenediamine. Highly porous sponge composed of chitosan (CS) and galactosylated hyaluronic acid (GHA) was prepared by freezing-drying technique. The morphology of the scaffolds was observed via scanning electron microscopy. Porosity and pore size of the sponge were greatly dependent on the content of GHA and freezing temperature. The addition of GHA not only improved the wettability and changed their mechanical properties, but also significantly influenced the cell attachment ratio. Moreover, liver functions of the hepatocytes such as albumin secretion, urea synthesis and ammonia elimination in the CS/GHA scaffolds were improved in comparison with those in the chitosan scaffolds.
肝细胞特异性三维组织工程支架在生物人工肝脏设备的开发中起着重要作用。在本研究中，通过乙二胺将半乳糖基共价偶联到透明质酸上。通过冷冻干燥技术制备了由壳聚糖（CS）和半乳糖化透明质酸（GHA）组成的高多孔海绵。通过扫描电子显微镜观察支架的形态。海绵的孔隙率和孔径大小极大地取决于 GHA 的含量和冷冻温度。添加 GHA 不仅提高了润湿性并改变了它们的机械性能，而且还显著影响了细胞附着率。此外，与壳聚糖支架相比，CS/GHA 支架中的肝细胞的白蛋白分泌、尿素合成和氨消除等肝功能得到了改善。
