Verschoor Chris P, Pant Sameer D, Schenkel Flavio S, Sharma Bhawani S, Karrow Niel A
Department of Animal and Poultry Science, Centre for Genetic Improvement of Livestock, University of Guelph, Guelph, N1G 2W1, ON, Canada.
Mamm Genome. 2009 Jul;20(7):447-54. doi: 10.1007/s00335-009-9198-1. Epub 2009 Jul 30.
Altering the balance between pro- and anti-inflammatory responses can influence an animal's susceptibility to acute or chronic inflammatory disease; bovine mastitis is no exception. Genetic variation in the form of single nucleotide polymorphisms (SNPs) may alter the function and expression of genes that regulate inflammation, making them important candidates for defining an animal's risk of developing acute or chronic mastitis. The objective of the present study was to identify SNPs in genes that regulate anti-inflammatory responses and test their association with estimated breeding values (EBVs) for somatic cell score (SCS), a trait highly correlated with the incidence of mastitis. These genes included bovine interleukin-10 (IL-10) and its receptor (IL-10R), and transforming growth factor beta1 (TGF-beta1) and its receptor (TGF-betaR). Sequencing-pooled DNA allowed for the identification of SNPs in IL-10 (n = 2), IL-10Ralpha (n = 6) and beta (n = 2), and TGF-beta1 (n = 1). These SNPs were subsequently genotyped in a cohort of Holstein (n = 500), Jersey (n = 83), and Guernsey (n = 50) bulls. Linear regression analysis identified significant SNP effects for IL-10Ralpha 1185C>T with SCS. Haplotype IL-10Ralpha AAT showed a significant effect on increasing SCS compared to the most common haplotype. The results presented here indicate that SNPs in IL-10Ralpha may contribute to variation in the SCS of dairy cattle. Although functional studies are necessary to ascertain whether these SNPs are causal polymorphisms or merely in linkage with the true causal SNP(s), a selection program incorporating these markers could have a beneficial influence on the average SCS and productivity of a dairy herd.
改变促炎反应和抗炎反应之间的平衡会影响动物对急性或慢性炎症性疾病的易感性；牛乳腺炎也不例外。单核苷酸多态性（SNP）形式的基因变异可能会改变调节炎症的基因的功能和表达，使其成为定义动物患急性或慢性乳腺炎风险的重要候选因素。本研究的目的是鉴定调节抗炎反应的基因中的SNP，并测试它们与体细胞评分（SCS）的估计育种值（EBV）的关联，SCS是一种与乳腺炎发病率高度相关的性状。这些基因包括牛白细胞介素-10（IL-10）及其受体（IL-10R），以及转化生长因子β1（TGF-β1）及其受体（TGF-βR）。对混合DNA进行测序可鉴定出IL-10（n = 2）、IL-10Rα（n = 6）和β（n = 2）以及TGF-β1（n = 1）中的SNP。随后在一群荷斯坦（n = 500）、泽西（n = 83）和根西（n = 50）公牛中对这些SNP进行基因分型。线性回归分析确定IL-10Rα 1185C>T与SCS存在显著的SNP效应。与最常见的单倍型相比，单倍型IL-10Rα AAT对增加SCS有显著影响。此处呈现的结果表明，IL-10Rα中的SNP可能导致奶牛SCS的变异。尽管需要进行功能研究以确定这些SNP是因果多态性还是仅仅与真正的因果SNP连锁，但纳入这些标记的选择计划可能会对奶牛群的平均SCS和生产力产生有益影响。
