牛 IGF2、LEP 和 CCL2 基因在肝脏、肾脏和垂体中的等位基因表达失衡。
Allelic gene expression imbalance of bovine IGF2, LEP and CCL2 genes in liver, kidney and pituitary.
机构信息
Department of Molecular Biology, Institute of Genetics and Animal Breeding, Polish Academy of Sciences (IGAB PAS), Jastrzębiec, 05-552, Magdalenka, Poland.
出版信息
Mol Biol Rep. 2013 Feb;40(2):1189-200. doi: 10.1007/s11033-012-2161-3. Epub 2012 Nov 25.
Abstract
Allelic expression imbalance (AEI) is an important genetic factor being the cause of differences in phenotypic traits that can be heritable. Studying AEI can be useful in searching for factors that modulate gene expression and help to understand molecular mechanisms underlying phenotypic changes. Although it was commonly recognized in many species and we know many genes show allelic expression imbalance, this phenomena was not studied on a larger scale in cattle. Using the pyrosequencing method we analyzed a set of 29 bovine genes in order to find those that have preferential allelic expression. The study was conducted in three tissues: liver, pituitary and kindey. Out of the studied group of genes 3 of them-LEP (leptin), IGF2 (insulin-like growth factor 2), CCL2 (chemokine C-C motif ligand 2) showed allelic expression imbalance.
摘要
等位基因表达失衡（AEI）是一个重要的遗传因素，导致表型性状的差异具有遗传性。研究 AEI 可以帮助寻找调节基因表达的因素，并有助于理解表型变化的分子机制。尽管在许多物种中普遍存在，并且我们知道许多基因表现出等位基因表达失衡，但在牛中尚未在更大范围内研究这种现象。本研究使用焦磷酸测序方法分析了 29 个牛基因，以寻找具有优先等位基因表达的基因。研究在三个组织中进行：肝脏、垂体和肾脏。在所研究的基因中，有 3 个基因（LEP、IGF2 和 CCL2）表现出等位基因表达失衡。
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