Watanabe K, Kinoshita S, Nakagawa H
Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
Nephron. 1990;56(4):405-9. doi: 10.1159/000186184.
A significant increase in gelatinolytic activity was observed in cultures of glomeruli from Heymann nephritic rats. Zymography of the culture medium indicated that the main gelatinase species has a molecular weight of 98 kilodaltons (kDa) and the characteristics of metalloproteinase. The 98-kDa gelatinase was detected in the culture medium of glomerular epithelial cells but not in those of endothelial and mesangial cells. Interleukin-1 beta, tumor necrosis factor-alpha and lipopolysaccharide dose-dependently increased gelatinase production by epithelial cells. These results suggest that the gelatinase secreted by cytokine-stimulated glomerular epithelial cells participates in the pathological process of Heymann nephritis.
在海曼肾炎大鼠的肾小球培养物中观察到明胶酶活性显著增加。培养基的酶谱分析表明，主要的明胶酶种类分子量为98千道尔顿（kDa），具有金属蛋白酶的特性。在肾小球上皮细胞的培养基中检测到98-kDa明胶酶，而在内皮细胞和系膜细胞的培养基中未检测到。白细胞介素-1β、肿瘤坏死因子-α和脂多糖剂量依赖性地增加上皮细胞的明胶酶产生。这些结果表明，细胞因子刺激的肾小球上皮细胞分泌的明胶酶参与了海曼肾炎的病理过程。
