Yamabe H, Yoshikawa S, Ohsawa H, Inuma H, Miyata M, Sasaki T, Kaizuka M, Tamura N, Onodera K
Second Department of Internal Medicine, Hirosaki University School of Medicine, Japan.
Nephrol Dial Transplant. 1993;8(6):519-23. doi: 10.1093/ndt/8.6.519.
It is well known that fibrin deposition in Bowman's space in association with crescent formation may play an important role in progressive glomerular injury in crescentic glomerulonephritis. Recent reports describe the presence of a procoagulant activity (PCA) in the glomeruli and its increased expression in human and experimental nephritis. The cells that synthesize PCA have not yet been identified. We attempted to determine if glomerular epithelial cells (GEC), one of the prominent cell populations in the crescent, can produce PCA. The PCA of cultured rat GEC was measured by clotting and amidolytic assays. The cultured GEC yielded PCA with the characteristics of a tissue factor, and this PCA was stimulated by interleukin 1, tumour necrosis factor-alpha, and lipopolysaccharide. We concluded that GEC produce tissue-factor-like PCA and thereby may contribute to fibrin deposition, which, along with macrophage or monocyte infiltration, leads to crescent formation in crescentic glomerulonephritis.
众所周知，与新月体形成相关的鲍曼间隙中纤维蛋白沉积可能在新月体性肾小球肾炎的进行性肾小球损伤中起重要作用。最近的报告描述了肾小球中促凝活性（PCA）的存在及其在人类和实验性肾炎中的表达增加。合成PCA的细胞尚未确定。我们试图确定新月体中突出的细胞群体之一——肾小球上皮细胞（GEC）是否能产生PCA。通过凝血和酰胺分解测定法测量培养的大鼠GEC的PCA。培养的GEC产生具有组织因子特征的PCA，并且这种PCA受到白细胞介素1、肿瘤坏死因子-α和脂多糖的刺激。我们得出结论，GEC产生组织因子样PCA，从而可能导致纤维蛋白沉积，这与巨噬细胞或单核细胞浸润一起，导致新月体性肾小球肾炎中新月体的形成。
