Liu T, Gibbons R J, Hay D I
Department of Microbiology, Forsyth Dental Center, Boston, MA 02115.
Oral Microbiol Immunol. 1990 Jun;5(3):143-8. doi: 10.1111/j.1399-302x.1990.tb00412.x.
Strains of Streptococcus cricetus and Streptococcus rattus exhibited striking differences in their ability to bind to different types of collagen. For example, S. cricetus AHT bound in highest numbers to hydroxyapatite (HA) treated with human type V collagen, while rat type I collagen was ineffective. In contrast, human type V collagen was least effective in promoting attachment of S. rattus LB-1, while treatment with rat or human type I collagen was effective. Adsorption of both species to human type I collagen-treated HA showed a high correlation with a Langmuir model. Estimates of adsorption parameters indicated there were greater numbers of binding sites with higher affinity for S. rattus LB-1 than for S. cricetus AHT. Treatment of HA with either the alpha 1 (1) or alpha 2 (1) polypeptide chains of collagen was effective in promoting adhesion of S. rattus LB-1 cells. In contrast, the alpha 2 (1) chain was more effective than the alpha 1 (1), chain for S. cricetus AHT. These observations indicate that S. cricetus AHT and S. rattus LB-1 cells bind to different segments of collagen molecules. Adhesion of both species was also promoted by collagen-rich fractions of human dentin.
仓鼠链球菌和大鼠链球菌菌株在结合不同类型胶原蛋白的能力上表现出显著差异。例如，仓鼠链球菌AHT与经人V型胶原蛋白处理的羟基磷灰石（HA）结合数量最多，而大鼠I型胶原蛋白则无效。相反，人V型胶原蛋白在促进大鼠链球菌LB - 1附着方面效果最差，而用大鼠或人I型胶原蛋白处理则有效。两种菌株对人I型胶原蛋白处理的HA的吸附与朗缪尔模型高度相关。吸附参数估计表明，大鼠链球菌LB - 1比仓鼠链球菌AHT具有更多亲和力更高的结合位点。用胶原蛋白的α1（I）或α2（I）多肽链处理HA可有效促进大鼠链球菌LB - 1细胞的黏附。相比之下，对于仓鼠链球菌AHT，α2（I）链比α1（I）链更有效。这些观察结果表明，仓鼠链球菌AHT和大鼠链球菌LB - 1细胞结合到胶原蛋白分子的不同片段上。富含胶原蛋白的人牙本质部分也促进了两种菌株的黏附。
