Visca P, Berlutti F, Vittorioso P, Dalmastri C, Thaller M C, Valenti P
Istituto di Microbiologia, Facoltà di Medicina e Chirurgia, Università La Sapienza, Roma, Italy.
Med Microbiol Immunol. 1989;178(2):69-79. doi: 10.1007/BF00203302.
The growth of Streptococcus mutans 6715-13 in a rich medium (Todd Hewitt broth) was drastically reduced by addition of apo-lactoferrin (apo-Lf); this effect was bacteriostatic and reversible by saturation of Lf with iron. The influence of Lf, salivary proteins (SP) and bovine serum albumin (BSA) on the attachment of Streptococcus mutans to hydroxyapatite (HA) was successively investigated. Sorption of Lf, SP, and BSA to HA was dependent on the protein concentration and reached the end-point at about 80 mg of proteins per gram of HA. Similarly, the number of streptococci adsorbed to HA was correlated to the amount of cells available up to at least 10(7) cells per mg of HA. The adsorption of Lf, SP and BSA on HA reduced the number of attaching S. mutans cells. In particular, SP reduced the adsorption of S. mutans by 30%, whereas pre-coating of HA with apo- or iron-saturated Lf resulted in a three orders of magnitude reduction of S. mutans adsorption to HA, as demonstrated by means of different experimental procedures. The powerful adherence-inhibiting effect of apo-Lf together with its noticeable antibacterial activity towards S. mutans points to a biological significance of these phenomena also in vivo.
在丰富培养基（托德 - 休伊特肉汤）中，添加脱铁乳铁蛋白（apo-Lf）会使变形链球菌6715 - 13的生长显著降低；这种作用是抑菌性的，并且通过用铁使乳铁蛋白饱和可逆转。依次研究了乳铁蛋白（Lf）、唾液蛋白（SP）和牛血清白蛋白（BSA）对变形链球菌附着于羟基磷灰石（HA）的影响。Lf、SP和BSA在HA上的吸附取决于蛋白质浓度，每克HA吸附约80毫克蛋白质时达到吸附终点。同样，吸附到HA上的链球菌数量与每毫克HA至少10⁷个细胞的可用细胞数量相关。Lf、SP和BSA在HA上的吸附减少了附着的变形链球菌细胞数量。特别是，SP使变形链球菌的吸附减少了30%，而用脱铁或铁饱和的Lf预包被HA导致变形链球菌对HA的吸附减少了三个数量级，这通过不同的实验方法得到了证明。脱铁乳铁蛋白强大的黏附抑制作用及其对变形链球菌显著的抗菌活性表明这些现象在体内也具有生物学意义。
