Huang Yong, Shi Ming, Zhao Shulin
College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, China.
J Sep Sci. 2009 Sep;32(17):3001-6. doi: 10.1002/jssc.200900026.
A microchip electrophoresis (MCE) method with LIF detection was presented for quantification of D-aspartic acid (D-Asp) and D-glutamate (D-Glu) in biological samples. D-Asp and D-Glu were determined after precolumn derivatization with FITC. The chiral separation was performed on a glass/PDMS hybrid microfluidic chip using gamma-CD as chiral selector in the running buffer. High sensitive detection was obtained by the LIF detection. The LODs (S/N = 3) for D-Asp and D-Glu were 6.0x10(-8) and 4.0x10(-8) M, respectively. Using this method, the levels of D-Asp and D-Glu in rat brain and human cerebrospinal fluid (CSF) were determined.
提出了一种采用激光诱导荧光（LIF）检测的微芯片电泳（MCE）方法，用于定量生物样品中的D-天冬氨酸（D-Asp）和D-谷氨酸（D-Glu）。D-Asp和D-Glu在经异硫氰酸荧光素（FITC）进行柱前衍生化后进行测定。在手性分离过程中，以γ-环糊精（γ-CD）作为手性选择剂加入运行缓冲液，在玻璃/聚二甲基硅氧烷（PDMS）混合微流控芯片上进行。通过LIF检测实现了高灵敏度检测。D-Asp和D-Glu的检测限（S/N = 3）分别为6.0×10⁻⁸和4.0×10⁻⁸ M。利用该方法测定了大鼠脑和人脑脊液（CSF）中D-Asp和D-Glu的含量。
